用单克隆抗体酶免疫测定法鉴定和分型单纯疱疹病毒
Identification and typing of herpes simplex virus by enzyme immunoassay with monoclonal antibodies.
作者信息
Frame B, Mahony J B, Balachandran N, Rawls W E, Chernesky M A
出版信息
J Clin Microbiol. 1984 Aug;20(2):162-6. doi: 10.1128/jcm.20.2.162-166.1984.
Abstract
A double-antibody enzyme immunoassay was developed for the identification and typing of herpes simplex virus (HSV) by employing a polyclonal rabbit capture antiserum together with type-common and type 2-specific monoclonal antibodies as detectors. The test successfully identified 45 type I isolates and 30 type 2 isolates as HSVs. Compared with immunofluorescent staining and restriction endonuclease analysis, enzyme immunoassay correctly typed 45 type 1 and 30 type 2 HSV isolates. Enzyme immunoassay was 100% sensitive for identification of HSV as compared with cell culture and 100% specific for typing as compared with immunofluorescence and restriction endonuclease analysis. Electron microscopy analysis suggested that approximately 10(6) virus particles were required for the identification and typing of HSV by enzyme immunoassay.
摘要
开发了一种双抗体酶免疫测定法，通过使用多克隆兔捕获抗血清以及共同型和2型特异性单克隆抗体作为检测剂来鉴定单纯疱疹病毒（HSV）并进行分型。该试验成功地将45株1型分离株和30株2型分离株鉴定为HSV。与免疫荧光染色和限制性内切酶分析相比，酶免疫测定法正确地对45株1型和30株2型HSV分离株进行了分型。与细胞培养相比，酶免疫测定法对HSV鉴定的敏感性为100%，与免疫荧光和限制性内切酶分析相比，对分型的特异性为100%。电子显微镜分析表明，通过酶免疫测定法鉴定和分型HSV大约需要10(6)个病毒颗粒。
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