与使用（E）-5-（2-溴乙烯基）-2'-脱氧尿苷进行单纯疱疹病毒分型相关的问题。
Problems associated with the use of (E)-5-(2-bromovinyl)-2'-deoxyuridine for typing herpes simplex virus.
作者信息
Swierkosz E M, Arens M Q, Rivetna K A
出版信息
J Clin Microbiol. 1985 Mar;21(3):459-61. doi: 10.1128/jcm.21.3.459-461.1985.
Abstract
When 0.5 microgram of (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVdU) per ml was incorporated directly into cell culture medium inoculated with eight known positive specimens, one herpes simplex virus type 1 (HSV-1) isolate grew in the presence of BVdU and was misidentified. By plaque assay, the titers of 15 HSV-1 strains were reduced by more than 3 log10 by BVdU, and the titers of 16 HSV-2 strains were reduced by less than 2 log10. Titers of HSV-1 acyclovir-resistant strains were reduced by less than 1.5 log10, which was characteristic of HSV-2 strains. Thus, typing of HSV isolates in the presence of BVdU by plaque assay is reliable only if information regarding previous antiviral therapy is obtained.
摘要
当每毫升含0.5微克的（E）-5-（2-溴乙烯基）-2'-脱氧尿苷（BVdU）直接加入接种了8个已知阳性标本的细胞培养基中时，一株单纯疱疹病毒1型（HSV-1）分离株在BVdU存在的情况下生长并被误鉴定。通过空斑试验，15株HSV-1毒株的滴度被BVdU降低了超过3个对数10，而16株HSV-2毒株的滴度降低了不到2个对数10。HSV-1阿昔洛韦耐药毒株的滴度降低不到1.5个对数10，这是HSV-2毒株的特征。因此，只有在获得有关先前抗病毒治疗的信息时，通过空斑试验在BVdU存在的情况下对HSV分离株进行分型才是可靠的。
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