用单克隆抗体对两种免疫荧光法进行单纯疱疹病毒分型的评估。
Evaluation of two immunofluorescence assays with monoclonal antibodies for typing of herpes simplex virus.
作者信息
Swierkosz E M, Arens M Q, Schmidt R R, Armstrong T
出版信息
J Clin Microbiol. 1985 Apr;21(4):643-4. doi: 10.1128/jcm.21.4.643-644.1985.
Abstract
An indirect immunofluorescence assay and a direct immunofluorescence assay were evaluated for typing clinical isolates of herpes simplex virus (HSV). The indirect immunofluorescence assay (Electro-Nucleonics, Inc.) correctly identified 16 HSV type 2 (HSV-2) isolates, but failed to identify 4 of 14 HSV-1 isolates because of background fluorescence and instability of reagents. Forty-nine HSV-1 isolates were correctly typed by direct immunofluorescence assay (Kallestad Laboratories, Inc.), but 1 of 39 HSV-2 isolates did not react with the HSV-2 type-specific antibody conjugate.
摘要
对间接免疫荧光测定法和直接免疫荧光测定法进行了评估，以对单纯疱疹病毒（HSV）的临床分离株进行分型。间接免疫荧光测定法（Electro-Nucleonics公司）正确鉴定出16株2型单纯疱疹病毒（HSV-2）分离株，但由于背景荧光和试剂不稳定，未能鉴定出14株HSV-1分离株中的4株。通过直接免疫荧光测定法（Kallestad Laboratories公司）正确分型了49株HSV-1分离株，但39株HSV-2分离株中有1株未与HSV-2型特异性抗体结合物发生反应。
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