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用单克隆抗体对两种免疫荧光法进行单纯疱疹病毒分型的评估。

Evaluation of two immunofluorescence assays with monoclonal antibodies for typing of herpes simplex virus.

作者信息

Swierkosz E M, Arens M Q, Schmidt R R, Armstrong T

出版信息

J Clin Microbiol. 1985 Apr;21(4):643-4. doi: 10.1128/jcm.21.4.643-644.1985.

DOI:10.1128/jcm.21.4.643-644.1985
PMID:2985651
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC271739/
Abstract

An indirect immunofluorescence assay and a direct immunofluorescence assay were evaluated for typing clinical isolates of herpes simplex virus (HSV). The indirect immunofluorescence assay (Electro-Nucleonics, Inc.) correctly identified 16 HSV type 2 (HSV-2) isolates, but failed to identify 4 of 14 HSV-1 isolates because of background fluorescence and instability of reagents. Forty-nine HSV-1 isolates were correctly typed by direct immunofluorescence assay (Kallestad Laboratories, Inc.), but 1 of 39 HSV-2 isolates did not react with the HSV-2 type-specific antibody conjugate.

摘要

对间接免疫荧光测定法和直接免疫荧光测定法进行了评估,以对单纯疱疹病毒(HSV)的临床分离株进行分型。间接免疫荧光测定法(Electro-Nucleonics公司)正确鉴定出16株2型单纯疱疹病毒(HSV-2)分离株,但由于背景荧光和试剂不稳定,未能鉴定出14株HSV-1分离株中的4株。通过直接免疫荧光测定法(Kallestad Laboratories公司)正确分型了49株HSV-1分离株,但39株HSV-2分离株中有1株未与HSV-2型特异性抗体结合物发生反应。

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本文引用的文献

1
Incorrect typing of herpes simplex virus isolates by direct immunofluorescence.通过直接免疫荧光法对单纯疱疹病毒分离株进行错误分型。
J Infect Dis. 1983 Aug;148(2):338. doi: 10.1093/infdis/148.2.338.
2
Simplified method for typing herpes simplex virus by restriction endonuclease analysis.通过限制性内切酶分析对单纯疱疹病毒进行分型的简化方法。
J Clin Microbiol. 1983 Mar;17(3):548-51. doi: 10.1128/jcm.17.3.548-551.1983.
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Comparison of biological, biochemical, immunological, and immunochemical techniques for typing herpes simplex virus isolates.用于单纯疱疹病毒分离株分型的生物学、生物化学、免疫学及免疫化学技术比较
J Clin Microbiol. 1983 Mar;17(3):396-9. doi: 10.1128/jcm.17.3.396-399.1983.
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Serological analysis of herpes simplex virus types 1 and 2 with monoclonal antibodies.用单克隆抗体对1型和2型单纯疱疹病毒进行血清学分析。
Infect Immun. 1982 Jan;35(1):363-7. doi: 10.1128/iai.35.1.363-367.1982.
5
Fc receptors induced by herpes simplex virus. I. Biologic and biochemical properties.单纯疱疹病毒诱导的Fc受体。I.生物学和生化特性。
J Immunol. 1978 Aug;121(2):726-30.