Reiss C S, Evans G A, Margulies D H, Seidman J G, Burakoff S J
Proc Natl Acad Sci U S A. 1983 May;80(9):2709-12. doi: 10.1073/pnas.80.9.2709.
In order to identify the site(s) on major histocompatibility molecules recognized by cytolytic T lymphocytes (CTLs), the recognition of H-2 antigens expressed when cloned genes are introduced into mouse L cells by DNA-mediated gene transfer has come under investigation. Recently, recombinant H-2 genes have been constructed in vitro from restriction endonuclease fragments of cloned H-2Dd and H-2Ld genes which exchange the N and C1 external domains (exon shuffling). These hybrid H-2 genes direct the synthesis of hybrid H-2 antigens when introduced into L cells by DNA-mediated gene transfer. These transformed L cells have been used as target cells to achieve a more precise localization of the sites recognized by allospecific and virus-specific CTLs. CTL systems were chosen that allow one to probe allospecific Ld or Dd recognition or virus-restricted Ld or Dd recognition. Using this approach we were able to map essential CTL recognition sites to the N and C1 domains of class 1 molecules.
为了确定细胞毒性T淋巴细胞（CTL）识别的主要组织相容性分子上的位点，通过DNA介导的基因转移将克隆基因导入小鼠L细胞时表达的H-2抗原的识别情况已受到研究。最近，已从克隆的H-2Dd和H-2Ld基因的限制性内切酶片段体外构建了重组H-2基因，这些片段交换了N和C1外部结构域（外显子改组）。当通过DNA介导的基因转移将这些杂交H-2基因导入L细胞时，它们指导杂交H-2抗原的合成。这些转化的L细胞已用作靶细胞，以更精确地定位同种特异性和病毒特异性CTL识别的位点。选择了CTL系统，以便能够探究同种特异性Ld或Dd识别或病毒限制的Ld或Dd识别。使用这种方法，我们能够将关键的CTL识别位点定位到1类分子的N和C1结构域。
