Raunio H, Vähäkangas K, Saarni H, Pelkonon O
Acta Pharmacol Toxicol (Copenh). 1983 Mar;52(3):168-74. doi: 10.1111/j.1600-0773.1983.tb01081.x.
The response of rat lung and liver ornithine decarboxylase (ODC) and aryl hydrocarbon hydroxylase (AHH) activities and lung benzo(a)pyrene (BP) metabolism was studied after exposing the rats to cigarette smoke. A close analysis of the time curves for ODC and AHH activities in rat lung and liver after a single exposure to cigarette smoke resulted in no clear correlation between the two parameters. Prolonged treatment (10 days) produced an increase in pulmonary ODC activity; hepatic ODC activity was unaffected. 10-day treatment was ineffective in raising AHH activity above values observed after a single treatment. BP metabolism, as determined in isolated perfused lungs by the appearance of organic- and water-soluble metabolites in the perfusion medium, the amount of covalently bound metabolites in lung tissue and the disappearance of unchanged 3-H BP from the perfusate, was markedly increased in response to cigarette smoke treatment. The data presented indicate that induction of AHH activity and increased metabolism of BP do not necessarily require a pre-existing increase in ODC activity.
在将大鼠暴露于香烟烟雾后，研究了大鼠肺和肝中鸟氨酸脱羧酶（ODC）和芳烃羟化酶（AHH）的活性以及肺中苯并（a）芘（BP）的代谢情况。对单次暴露于香烟烟雾后大鼠肺和肝中ODC和AHH活性的时间曲线进行仔细分析后发现，这两个参数之间没有明显的相关性。长时间处理（10天）使肺中ODC活性增加；肝中ODC活性未受影响。10天的处理在将AHH活性提高到单次处理后观察到的值以上方面无效。通过灌注介质中有机和水溶性代谢物的出现、肺组织中共价结合代谢物的量以及灌注液中未改变的3-H BP的消失来确定的BP代谢，在香烟烟雾处理后明显增加。所呈现的数据表明，AHH活性的诱导和BP代谢的增加不一定需要预先存在的ODC活性增加。
