使用大肠杆菌突变菌株可测定粗提物中的单链脱嘌呤-脱嘧啶内切核酸酶：对未转化细胞和用含uvrC基因的质粒转化的细胞的研究。
Use of an Escherichia coli mutant strain permits measurement of single-stranded apurinic-apyrimidinic endonuclease in crude extracts: studies with untransformed cells and cells transformed with plasmids containing the uvrC gene.
作者信息
Schultz R A, Friedberg E C, Moses R E, Rupp W D, Sancar A, Sharma S
出版信息
J Bacteriol. 1983 Jun;154(3):1459-61. doi: 10.1128/jb.154.3.1459-1461.1983.
Abstract
We have constructed a strain of Escherichia coli that is defective in exonuclease VII and uracil-DNA glycosylase activities. This strain (xse ung) facilitates the quantitation of single-stranded apurinic-apyrimidinic endonuclease activity in crude extracts. Quantitative comparisons of single-stranded apurinic-apyrimidinic endonuclease activity under conditions in which uvrC protein is overexpressed showed no differences, suggesting that single-stranded apurinic-apyrimidinic endonuclease and uvrC protein are probably distinct.
摘要
我们构建了一种在核酸外切酶VII和尿嘧啶-DNA糖基化酶活性方面存在缺陷的大肠杆菌菌株。该菌株（xse ung）有助于对粗提物中的单链脱嘌呤-脱嘧啶内切酶活性进行定量分析。在uvrC蛋白过表达的条件下，对单链脱嘌呤-脱嘧啶内切酶活性进行的定量比较未显示出差异，这表明单链脱嘌呤-脱嘧啶内切酶和uvrC蛋白可能是不同的。
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