Takamatsu N, Ohno T, Meshi T, Okada Y
Nucleic Acids Res. 1983 Jun 11;11(11):3767-78. doi: 10.1093/nar/11.11.3767.
The cDNA copies of tobacco mosaic virus (TMV)-tomato strain (L) genome were cloned by the method of Okayama and Berg (Mol. Cell. Biol. 2, 161-170. (1982)) and the sequence of 1,614 nucleotides at the 3' end was determined. The sequence encompasses the 30K and the coat protein cistron which are located in residues 685-1, 479 and 203-682 from the 3' end of the genome respectively. The close relationship between the tomato and the common strain was shown on the level of the nucleotide sequence. Highly homologous regions are found in the 3' non-coding region, the assembly origin and the 5' flanking region of the 30K protein cistron. The comparison of the deduced amino acid sequence between the tomato and the common strain shows that the 30K protein is composed of the conserved N-terminal four-fifth and the highly divergent region near the C-terminus.
采用冈山县和伯格的方法(《分子细胞生物学》2, 161 - 170. (1982))克隆了烟草花叶病毒(TMV)番茄株系(L)基因组的cDNA拷贝,并测定了3'端1614个核苷酸的序列。该序列包含分别位于基因组3'端第685 - 1479位残基和第203 - 682位残基的30K蛋白编码区和外壳蛋白编码区。在核苷酸序列水平上显示了番茄株系和普通株系之间的密切关系。在3'非编码区、装配起始位点和30K蛋白编码区的5'侧翼区发现了高度同源区域。番茄株系和普通株系推导的氨基酸序列比较表明,30K蛋白由保守的N端五分之四和C端附近高度不同的区域组成。