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猿猴病毒40 DNA复制：调控元件的功能组织

Simian virus 40 DNA replication: functional organization of regulatory elements.

作者信息

Lee-Chen G J, Woodworth-Gutai M

出版信息

Mol Cell Biol. 1986 Sep;6(9):3086-93. doi: 10.1128/mcb.6.9.3086-3093.1986.

DOI:10.1128/mcb.6.9.3086-3093.1986
PMID:3023962
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC367043/
Abstract

The efficiency of simian virus 40 (SV40) DNA replication is dependent on the structural organization of the regulatory region. The enhancing effect of the G + C-rich 21-base-pair (bp) repeats on SV40 DNA replication is position and dose dependent and to some extent orientation dependent. The inverted orientation is about 50% as effective as the normal orientation of the 21-bp repeat region. Movement of the 21-bp repeat region 180 or 370 bp upstream of the ori sequence abolishes its enhancing effect, whereas no replication is detected if the 21-bp repeat region is placed downstream of the ori sequence. The dose-dependent enhancement of the 21-bp repeat of SV40 DNA replication as first described in single transfection by Bergsma et al. (D. J. Bergsma, D. M. Olive, S. W. Hartzell, and K. N. Subramanian, Proc. Natl. Acad. Sci. USA 79:381-385, 1982) is dramatically amplified in mixed transfection. In the presence of the 21-bp repeat region, the 72-bp repeat region can enhance SV40 DNA replication. In the presence of the 21-bp repeats and a competitive environment, the 72-bp repeat region exhibits a cis-acting inhibitory effect on SV40 DNA replication.

摘要

猿猴病毒40（SV40）DNA复制的效率取决于调控区的结构组织。富含G + C的21碱基对（bp）重复序列对SV40 DNA复制的增强作用具有位置和剂量依赖性，并且在一定程度上具有方向依赖性。21 bp重复区域的反向方向的有效性约为正常方向的50%。将21 bp重复区域移至ori序列上游180或370 bp会消除其增强作用，而如果将21 bp重复区域置于ori序列下游，则检测不到复制。如Bergsma等人首次在单次转染中描述的那样（D. J. Bergsma、D. M. Olive、S. W. Hartzell和K. N. Subramanian，《美国国家科学院院刊》79:381 - 385，1982年），SV40 DNA复制的21 bp重复序列的剂量依赖性增强在混合转染中显著放大。在存在21 bp重复区域的情况下，72 bp重复区域可以增强SV40 DNA复制。在存在21 bp重复序列和竞争环境的情况下，72 bp重复区域对SV40 DNA复制表现出顺式作用抑制效应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b754/367043/7aefc86562ed/molcellb00093-0077-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b754/367043/8737d56de756/molcellb00093-0074-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b754/367043/4787b72930d6/molcellb00093-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b754/367043/65c5a7a6592f/molcellb00093-0075-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b754/367043/99bfa5a4bc02/molcellb00093-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b754/367043/7aefc86562ed/molcellb00093-0077-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b754/367043/8737d56de756/molcellb00093-0074-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b754/367043/4787b72930d6/molcellb00093-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b754/367043/65c5a7a6592f/molcellb00093-0075-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b754/367043/99bfa5a4bc02/molcellb00093-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b754/367043/7aefc86562ed/molcellb00093-0077-a.jpg

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1
Simian virus 40 DNA replication: functional organization of regulatory elements.猿猴病毒40 DNA复制：调控元件的功能组织
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Characterization of the simian virus 40 late promoter: relative importance of sequences within the 72-base-pair repeats differs before and after viral DNA replication.猴病毒40晚期启动子的特性：病毒DNA复制前后72碱基对重复序列内各序列的相对重要性有所不同。
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Nucleic Acids Res. 1981 Nov 25;9(22):6047-68. doi: 10.1093/nar/9.22.6047.

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The A+T-rich sequence of the simian virus 40 origin is essential for replication and is involved in bending of the viral DNA.猿猴病毒40复制起点富含A+T的序列对复制至关重要，且参与病毒DNA的弯曲。
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Mutational dissection of the 21 bp repeat region of the SV40 early promoter reveals that it contains overlapping elements of the early-early and late-early promoters.对SV40早期启动子21bp重复区域的突变分析表明，它包含早期-早期启动子和晚期-早期启动子的重叠元件。
Nucleic Acids Res. 1984 Jan 25;12(2):915-32. doi: 10.1093/nar/12.2.915.
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Simian virus 40 early- and late-region promoter functions are enhanced by the 72-base-pair repeat inserted at distant locations and inverted orientations.猿猴病毒40早期和晚期区域启动子功能可通过插入到远处位置且方向相反的72碱基对重复序列得到增强。
Mol Cell Biol. 1983 Jun;3(6):991-9. doi: 10.1128/mcb.3.6.991-999.1983.
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The repeated GC-rich motifs upstream from the TATA box are important elements of the SV40 early promoter.TATA框上游富含GC的重复基序是SV40早期启动子的重要元件。
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