Terkeltaub R, Tenner A J, Kozin F, Ginsberg M H
Arthritis Rheum. 1983 Jun;26(6):775-83. doi: 10.1002/art.1780260612.
Using 2-dimensional O'Farrell gel electrophoresis, we have mapped the proteins from undiluted plasma and serum which bind to monosodium urate (MSU) crystals. More than 30 crystal-associated polypeptides were visualized, including anionic and cationic species. Proteins increased on the crystals relative to plasma included C1q, C1-r, C1-s, fibronectin, fibrinogen, and kininogen. Crystal-bound polypeptides derived from IgG, albumin, and transferrin were recovered in decreased amounts relative to plasma. Direct evidence for activation of the complement and coagulation systems in plasma was provided by the identification of crystal-associated activation fragments of C1 and kininogen. Plasmas deficient in selected proteins (e.g., C1q and IgG) were used to define the role of these proteins in such activation events and confirmed activation of C1 in immunoglobulin-deficient plasma by MSU crystals. In summary, we have described a high resolution, semiquantitative approach to analyze protein binding to crystals, have documented the complexity of crystal-plasma protein interaction, and have provided direct evidence for the binding of coagulation system proteins and binding and activation of complement by MSU crystals, in whole plasma and IgG-deficient plasma.
我们使用二维O'Farrell凝胶电泳技术，对未稀释血浆和血清中与尿酸钠（MSU）晶体结合的蛋白质进行了图谱分析。观察到30多种与晶体相关的多肽，包括阴离子和阳离子种类。相对于血浆，晶体上增加的蛋白质包括C1q、C1-r、C1-s、纤连蛋白、纤维蛋白原和激肽原。相对于血浆，从IgG、白蛋白和转铁蛋白衍生的晶体结合多肽回收量减少。通过鉴定与晶体相关的C1和激肽原激活片段，为血浆中补体和凝血系统的激活提供了直接证据。使用缺乏特定蛋白质（如C1q和IgG）的血浆来确定这些蛋白质在这种激活事件中的作用，并证实MSU晶体在免疫球蛋白缺乏的血浆中激活C1。总之，我们描述了一种高分辨率、半定量的方法来分析蛋白质与晶体的结合，记录了晶体-血浆蛋白质相互作用的复杂性，并提供了凝血系统蛋白质结合以及MSU晶体在全血浆和IgG缺乏血浆中结合并激活补体的直接证据。
