Wilson V, Jeffreys A J, Barrie P A, Boseley P G, Slocombe P M, Easton A, Burke D C
J Mol Biol. 1983 Jun 5;166(4):457-75. doi: 10.1016/s0022-2836(83)80281-2.
Cloned human interferon complementary DNAs were used as hybridization probes to detect interferon alpha and beta gene families in restriction endonuclease digests of total genomic DNA isolated from a wide range of vertebrates and invertebrates. A complex interferon-alpha multigene family was detected in all mammals examined, whereas there was little or no cross-hybridization of human interferon-alpha complementary DNA to non-mammalian vertebrates or invertebrates. In contrast, human interferon-beta complementary DNA detected one or two interferon-beta genes in all mammals tested, with the exception of the cow and the blackbuck, both of which possessed a complex interferon-beta multigene family which has presumably arisen by a recent series of gene duplications. Interferon-beta sequences could also be detected in non-mammalian vertebrates ranging from birds to bony fish. Detailed restriction endonuclease mapping of DNA sequences neighbouring the interferon-beta gene in a variety of primates indicated a strong evolutionary conservation of flanking sequences, particularly on the 3' side of the gene.
克隆的人干扰素互补DNA被用作杂交探针，以检测从多种脊椎动物和无脊椎动物中分离出的总基因组DNA的限制性内切酶消化物中的干扰素α和β基因家族。在所有检测的哺乳动物中都检测到了一个复杂的干扰素α多基因家族，而人干扰素α互补DNA与非哺乳动物脊椎动物或无脊椎动物几乎没有或没有交叉杂交。相比之下，人干扰素β互补DNA在所有测试的哺乳动物中检测到一两个干扰素β基因，但牛和印度羚除外，这两种动物都拥有一个复杂的干扰素β多基因家族，推测这是最近一系列基因复制产生的。在从鸟类到硬骨鱼的非哺乳动物脊椎动物中也可以检测到干扰素β序列。对多种灵长类动物中与干扰素β基因相邻的DNA序列进行详细的限制性内切酶图谱分析表明，侧翼序列具有很强的进化保守性，尤其是在基因的3'端。
