Sehgal P B, Zilberstein A, Ruggieri R M, May L T, Ferguson-Smith A, Slate D L, Revel M, Ruddle F H
Proc Natl Acad Sci U S A. 1986 Jul;83(14):5219-22. doi: 10.1073/pnas.83.14.5219.
A cDNA clone (pAE20-4) corresponding to the 1.3-kilobase human beta 2 interferon mRNA was used as a probe in blot-hybridization experiments of DNA from a panel of human-rodent somatic cell hybrids containing overlapping subsets of human chromosomes. The DNA hybridization experiments showed that the human beta 2 interferon gene is located on human chromosome 7. This assignment is consistent with previous experimental data in which the expression of the translationally active 1.3-kilobase beta 2 interferon mRNA was assayed in various somatic cell hybrids. Blot-hybridization experiments using DNA from different human cell strains and cell lines reveal distinct EcoRI restriction fragment length polymorphisms of the human beta 2 interferon gene.
一个与1.3千碱基的人β2干扰素mRNA相对应的cDNA克隆（pAE20 - 4）被用作探针，用于对一组包含人类染色体重叠亚群的人 - 啮齿动物体细胞杂种的DNA进行印迹杂交实验。DNA杂交实验表明，人β2干扰素基因位于人类第7号染色体上。这一定位与先前的实验数据一致，在先前的实验中，对各种体细胞杂种中具有翻译活性的1.3千碱基β2干扰素mRNA的表达进行了检测。使用来自不同人类细胞株和细胞系的DNA进行的印迹杂交实验揭示了人β2干扰素基因不同的EcoRI限制性片段长度多态性。
