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Tn5插入到粘球菌中控制可刺激细胞运动性的基因附近。

Insertions of Tn5 near genes that govern stimulatable cell motility in Myxococcus.

作者信息

Sodergren E, Kaiser D

出版信息

J Mol Biol. 1983 Jun 25;167(2):295-310. doi: 10.1016/s0022-2836(83)80337-4.

Abstract

Insertions of transposon Tn5 were used to examine the genetics of motility mutants in Myxococcus. Fifteen independent insertions of Tn5 were isolated that were linked to seven different loci that govern motility. Among the motility mutants that can be stimulated to move transiently by contact with other cells, a one-to-one correspondence was confirmed between specificity of stimulation and genetic locus. There are six different specificities and six corresponding loci, as if each locus governs a different protein required for gliding motility.

摘要

转座子Tn5的插入被用于研究粘球菌运动突变体的遗传学。分离出了15个与7个控制运动的不同基因座连锁的Tn5独立插入突变体。在那些可通过与其他细胞接触而被短暂刺激移动的运动突变体中,刺激特异性与基因座之间的一一对应关系得到了证实。存在6种不同的特异性和6个相应的基因座,就好像每个基因座控制着滑行运动所需的一种不同蛋白质。

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