Williamson S E, Doolittle W F
Nucleic Acids Res. 1983 Jan 11;11(1):225-35. doi: 10.1093/nar/11.1.225.
A 6.3 kbp Eco RI-Bam HI fragment which carries most of one of the two rRNA gene clusters of the blue-green alga Anacystis nidulans was cloned into plasmid pBR322. Sequence analysis of the spacer region between the 16S and 23S rRNA genes reveals the presence of genes for tRNAIle and tRNAAla. The 16S rRNA gene is separated from the tRNAIle gene by a 162 bp spacer which shows significant homology to the comparable region in Zea mays plastids. The spacer between the two tRNA genes is 33 bp long and can be folded into a 9 bp stem and loop structure. The 5' portion of the tRNAIle gene is 60% homologous to a "pseudogene"-like sequence which maps beyond the 5S rRNA gene.
将蓝藻集胞藻6803两个rRNA基因簇之一的大部分基因所在的一段6.3kbp的EcoRI - BamHI片段克隆到质粒pBR322中。对16S和23S rRNA基因间间隔区的序列分析表明存在异亮氨酸tRNA和丙氨酸tRNA基因。16S rRNA基因与异亮氨酸tRNA基因被一个162bp的间隔区隔开,该间隔区与玉米质体中的相应区域具有显著同源性。两个tRNA基因间的间隔区长33bp,可折叠成一个9bp的茎环结构。异亮氨酸tRNA基因的5'部分与一个位于5S rRNA基因之外的“假基因”样序列有60%的同源性。