Nielsen M, Christensen L, Albrechtsen R
Acta Pathol Microbiol Immunol Scand A. 1983 Jul;91(4):257-64. doi: 10.1111/j.1699-0463.1983.tb02755.x.
The occurrence of the basement membrane (BM) glycoprotein laminin was investigated in 91 malignant breast tumors and in 359 axillary lymph nodes of which 70 contained metastases. The indirect double-layer immunoperoxidase technique was applied using rabbit anti-rat laminin. Positive cytoplasmic staining for laminin was found in all breast carcinomas. BM could be demonstrated around in situ carcinomas and in the higher differentiated carcinomas around islands of infiltrating tumor cells. Lymph node metastases resembled the primary tumors in the cytoplasmic staining reaction for laminin, but no BM staining was found. Tissue from 50 benign breast lesions showed cytoplasmic staining for laminin in both the secretory and the myoepithelial cells, but consistently weaker than in the carcinomas. BM was always present.
在91例乳腺恶性肿瘤及359个腋窝淋巴结（其中70个有转移灶）中研究了基底膜（BM）糖蛋白层粘连蛋白的出现情况。采用兔抗大鼠层粘连蛋白，应用间接双层免疫过氧化物酶技术。在所有乳腺癌中均发现层粘连蛋白呈阳性细胞质染色。在原位癌周围以及浸润性肿瘤细胞岛周围的高分化癌中可显示出BM。淋巴结转移灶在层粘连蛋白的细胞质染色反应方面类似于原发肿瘤，但未发现BM染色。50例乳腺良性病变组织的分泌细胞和肌上皮细胞中层粘连蛋白均呈细胞质染色，但始终比癌组织中的染色弱。BM始终存在。
