Yeh H H, Woodward D J
Neuropharmacology. 1983 May;22(5):629-39. doi: 10.1016/0028-3908(83)90155-7.
Norepinephrine (NE) may have a major role in modulating the responsiveness of Purkinje cells to the actions of other neurotransmitters in the cerebellum. Specifically, the catecholamine has been shown to enhance inhibitory responses of Purkinje cells to microiontophoretically applied gamma-aminobutyric acid (GABA). This noradrenergic facilitation of GABA has been shown recently to be mediated by beta-adrenergic receptors. The aim of this study was to characterize further the subtype of the beta-receptor involved. The results indicate that practolol, a selective beta-1 blocker, antagonized the NE-induced augmentation of Purkinje cell inhibitory responses to GABA. Zinterol, a selective beta-2 agonist, did not mimic the potentiating effect of NE. Furthermore, the noradrenergic facilitation of GABA-induced inhibition remained unaltered in cerebella subjected to neonatal X-irradiation. This procedure resulted in a decrease in the total cerebellar beta-receptor population yet left unchanged both the number of beta-1 receptors per cerebellum and the radio-resistant Purkinje cells. Together, the data presented here support the hypothesis that the noradrenergic facilitation of GABA-induced inhibition of Purkinje cells is mediated by the activation of cerebellar beta-1 adrenergic receptors located on the Purkinje cells.
去甲肾上腺素（NE）可能在调节浦肯野细胞对小脑内其他神经递质作用的反应性方面发挥主要作用。具体而言，已证明这种儿茶酚胺可增强浦肯野细胞对微量离子电泳施加的γ-氨基丁酸（GABA）的抑制反应。最近已表明，NE对GABA的这种去甲肾上腺素能促进作用是由β-肾上腺素能受体介导的。本研究的目的是进一步确定所涉及的β受体亚型。结果表明，选择性β-1阻滞剂心得宁可拮抗NE诱导的浦肯野细胞对GABA抑制反应的增强。选择性β-2激动剂辛特罗尔并未模拟NE的增强作用。此外，在接受新生儿X射线照射的小脑中，NE对GABA诱导的抑制的促进作用保持不变。该过程导致小脑β受体总数减少，但每个小脑的β-1受体数量和抗辐射的浦肯野细胞数量均未改变。总之，此处提供的数据支持以下假设：NE对GABA诱导的浦肯野细胞抑制的促进作用是由位于浦肯野细胞上的小脑β-1肾上腺素能受体的激活介导的。
