Fuller D J, Gerner E W
Radiat Res. 1983 Jul;95(1):124-9.
The polyamines putrescine, spermidine, and spermine have been shown to sensitize Chinese hamster ovary (CHO) cells to the cytotoxic effects of elevated temperatures. This sensitization occurs in the order spermine greater than spermidine greater than putrescine. A series of homologs of the diamine putrescine demonstrated little variance in the degree of potentiation until a chain length equivalent to that of spermidine was employed (1,8-diaminooctane). Two compounds bearing structural similarities to spermidine gave rather different results when combined with heat. Methylglyoxal bis(guanylhydrazone) (MGBG), an antiproliferative drug, sensitized cells to heat, while S-2-(3-aminopropylamino)-ethyl phosphoric acid (WR-2721), a radioprotector, had no measureable effect on 43 degrees C-induced cytotoxicity. Together, these results point out the primary importance of two terminal amino groups separated by either carbon, or carbon and nitrogen, atoms in an aliphatic chain in the observed sensitization of heat-induced cytotoxicity by polyamines. These data suggest specific dimensional characteristics of the presumed negatively charged structure(s) with which the polyamines are interacting to elicit this effect.
多胺腐胺、亚精胺和精胺已被证明可使中国仓鼠卵巢（CHO）细胞对高温的细胞毒性作用敏感。这种敏感性按精胺大于亚精胺大于腐胺的顺序发生。一系列二胺腐胺的同系物在增强程度上差异不大，直到使用与亚精胺链长相当的化合物（1,8 - 二氨基辛烷）。两种与亚精胺结构相似的化合物与热联合使用时产生了相当不同的结果。甲基乙二醛双（脒腙）（MGBG），一种抗增殖药物，使细胞对热敏感，而S - 2 -（3 - 氨丙基氨基） - 乙基磷酸（WR - 2721），一种辐射防护剂，对43℃诱导的细胞毒性没有可测量的影响。总之，这些结果指出了在脂肪链中被碳或碳和氮原子隔开的两个末端氨基在多胺对热诱导细胞毒性的观察到的敏感性中的首要重要性。这些数据表明了假定的带负电荷结构的特定尺寸特征，多胺与之相互作用以引发这种效应。
