van de Griend R J, van der Reijden H J, Bolhuis R L, Melief C J, von dem Borne A E, Roos D
Blood. 1983 Sep;62(3):669-76.
Lymphocytes from patients with acute and chronic T-cell malignancy or chronic T gamma lymphocytosis were characterized by studying the activity of three enzymes involved in purine metabolism and by determining the isoenzyme pattern of lactate dehydrogenase (LDH) in addition to analysis of surface marker expression with monoclonal antibodies. Four clinically different types of disease were distinguished on the basis of the enzyme parameters. Lymphocytes from patients with acute lymphocytic leukemia (T-ALL) showed an enzyme profile similar to that of normal thymocytes, i.e., an elevated level of adenosine deaminase (ADA) activity as compared with normal T lymphocytes, reduced activities of purine 5'nucleotidase (5'NT) and purine nucleoside phosphorylase (PNP), and a binomial distribution of the LDH isoenzyme pattern. Cells from "null"-ALL patients had an ADA/PNP ratio that was intermediate between that of normal T cells and that of T-ALL cells or thymocytes, but their 5'NT activity and LDH isoenzyme pattern were thymocyte-like. Patients with chronic T-cell proliferation were subdivided into those with chronic T gamma lymphocytosis and those with proven chronic T malignancy. The lymphocytes from these patients had ADA and PNP activities within the ranges of those of normal T lymphocytes. However, the ADA activity and/or the ADA/PNP ratio were consistently higher in the cells from the patients with chronic T gamma lymphocytosis than in those with chronic T malignancy. The enzyme profile of the cells from the T gamma patients was similar to that of T gamma cells of normal individuals. The cells from patients with chronic T malignancies showed a heterogeneous enzyme pattern as compared with that of normal T lymphocytes. Analysis with monoclonal antibodies enabled us to distinguish null-ALL patients from the other leukemias studied, but a distinction between chronic and acute T-cell proliferation disease, for instance, was not possible with monoclonal antibodies alone. Our data demonstrate that the enzyme profiles studied provide supplementary information for classification and diagnosis of lymphoproliferative diseases to that obtained with cell surface markers alone.
通过研究参与嘌呤代谢的三种酶的活性、测定乳酸脱氢酶（LDH）的同工酶谱，以及使用单克隆抗体分析表面标志物表达，对急性和慢性T细胞恶性肿瘤患者或慢性Tγ淋巴细胞增多症患者的淋巴细胞进行了特征分析。根据酶参数区分出四种临床不同类型的疾病。急性淋巴细胞白血病（T-ALL）患者的淋巴细胞酶谱与正常胸腺细胞相似，即与正常T淋巴细胞相比，腺苷脱氨酶（ADA）活性水平升高，嘌呤5'-核苷酸酶（5'NT）和嘌呤核苷磷酸化酶（PNP）活性降低，且LDH同工酶谱呈二项分布。“无”ALL患者的细胞ADA/PNP比值介于正常T细胞与T-ALL细胞或胸腺细胞之间，但它们的5'NT活性和LDH同工酶谱类似胸腺细胞。慢性T细胞增殖患者被细分为慢性Tγ淋巴细胞增多症患者和经证实的慢性T恶性肿瘤患者。这些患者的淋巴细胞ADA和PNP活性在正常T淋巴细胞的范围内。然而，慢性Tγ淋巴细胞增多症患者细胞中的ADA活性和/或ADA/PNP比值始终高于慢性T恶性肿瘤患者。Tγ患者细胞的酶谱与正常个体的Tγ细胞相似。与正常T淋巴细胞相比，慢性T恶性肿瘤患者的细胞显示出异质的酶谱。使用单克隆抗体分析使我们能够将无ALL患者与其他研究的白血病区分开来，但仅靠单克隆抗体无法区分慢性和急性T细胞增殖性疾病。我们的数据表明，所研究的酶谱为淋巴细胞增殖性疾病的分类和诊断提供了补充信息，补充了仅通过细胞表面标志物获得的信息。
