Maeda K, Ito K, Yamaguchi N
Blood. 1981 Nov;58(5):897-903.
New techniques have been devised for the cytochemical demonstration of purine nucleoside phosphorylase (PNP) and adenosine deaminase (ADA) activities in unfixed human lymphocytes. A suspension of living lymphocytes is mixed with agarose sol containing the reagents for the detection of PNP or ADA activity on a glass slide. The mixture solidifies, is incubated, and then dried for lightmicroscopic observation. Reactive cells are recognized by the diffusely deposited granules of formazan, the end-product of the cytochemical reaction, and are divided into three groups of the cell with the low, middle, and high enzyme activity by the number of the granule. In healthy adults, the mean percentages of PNP- and ADA-positive cells were more than 90% in unfractionated lymphocytes, T-cell fractions, and complement-receptor cell fractions and cells with middle PNP and ADA activities were predominant. The PNP and ADA staining was observed in lymphoid cells of patients with lymphoproliferative disorders. A decrease in the percentage of PNP-positive cells concomitant with a relative increase of cells with the low enzyme activity was observed in the lymphocytes of nine patients with chronic lymphocytic leukemia (CLL). Similar findings were obtained in the ADA staining of the lymphocytes of five patients with B-cell CLL.
已设计出新技术用于在未固定的人淋巴细胞中进行细胞化学方法显示嘌呤核苷磷酸化酶（PNP）和腺苷脱氨酶（ADA）的活性。将活淋巴细胞悬液与含有用于检测PNP或ADA活性试剂的琼脂糖溶胶在载玻片上混合。混合物凝固、孵育，然后干燥以供光学显微镜观察。反应性细胞通过细胞化学反应的终产物甲臜的弥漫性沉积颗粒来识别，并根据颗粒数量分为酶活性低、中、高的三组细胞。在健康成年人中，未分级淋巴细胞、T细胞组分和补体受体细胞组分中PNP和ADA阳性细胞的平均百分比超过90%，且具有中等PNP和ADA活性的细胞占主导。在淋巴增殖性疾病患者的淋巴细胞中观察到PNP和ADA染色。在9例慢性淋巴细胞白血病（CLL）患者的淋巴细胞中，观察到PNP阳性细胞百分比降低，同时酶活性低的细胞相对增加。在5例B细胞CLL患者的淋巴细胞ADA染色中也获得了类似结果。
