van Laarhoven J P, de Gast G C, Spierenburg G T, de Bruyn C H
Leuk Res. 1983;7(2):261-7. doi: 10.1016/0145-2126(83)90016-4.
Adenosine deaminase (ADA), 5'nucleotidase (5'NT), ecto-5'NT, purine nucleoside phosphorylase (PNP), hypoxanthine-guanine phosphoribosyltransferase (HGPRT), adenine phosphoribosyltransferase (APRT), adenosine kinase (AK), AMP-deaminase (AMPD) and adenylate kinase (AdKin) activities were assayed in peripheral blood lymphoid cells from 20 patients with B-cell type chronic lymphocytic leukemia (CLL). Significantly decreased mean activities of ADA, 5'NT, ecto-5'NT, PNP and AMPD were observed when comparing B-CLL lymphoid cells with control peripheral blood lymphocytes (PBL). AK and AdKin activities however, were found to be higher in B-CLL. Relatively wide ranges of ADA and 5'NT activity were observed. In patients with paraproteinaemia, 5'NT activity was found to be relatively high and in the range of the activities in normal PBL. ADA activity seemed to be slightly higher in patients without paraproteinaemia. No correlation could be found between the enzyme activities and the number of cells rosetting with sheep erythrocytes or bearing surface immunoglobulin (sIg). A relationship was suggested between 5'NT activity and Ig production.
对20例B细胞型慢性淋巴细胞白血病（CLL）患者外周血淋巴细胞中的腺苷脱氨酶（ADA）、5′核苷酸酶（5′NT）、胞外5′NT、嘌呤核苷磷酸化酶（PNP）、次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HGPRT）、腺嘌呤磷酸核糖转移酶（APRT）、腺苷激酶（AK）、AMP脱氨酶（AMPD）和腺苷酸激酶（AdKin）活性进行了检测。与对照外周血淋巴细胞（PBL）相比，B-CLL淋巴细胞中ADA、5′NT、胞外5′NT、PNP和AMPD的平均活性显著降低。然而，发现B-CLL中AK和AdKin活性较高。观察到ADA和5′NT活性的范围相对较宽。在副蛋白血症患者中，发现5′NT活性相对较高，且在正常PBL的活性范围内。无副蛋白血症患者的ADA活性似乎略高。酶活性与羊红细胞花环形成细胞数或表面免疫球蛋白（sIg）阳性细胞数之间未发现相关性。提示5′NT活性与Ig产生之间存在关系。
