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用于诊断轮状病毒肠胃炎的Rotazyme II酶免疫测定法的检测

Examination of the Rotazyme II enzyme immunoassay for the diagnosis of rotavirus gastroenteritis.

作者信息

Chernesky M, Castriciano S, Mahony J, DeLong D

出版信息

J Clin Microbiol. 1985 Sep;22(3):462-4. doi: 10.1128/jcm.22.3.462-464.1985.

Abstract

Rotazyme II, which is a shorter version of Rotazyme (less than 3 h), was compared with electron microscopy and Rotazyme for sensitivity and specificity on 229 human stool specimens. Compared with electron microscopy, the newer assay was found to be 99.4% sensitive and 97.3% specific for an overall agreement of 98.7%. After resolution of discordant results by blocking tests, the Rotazyme II enzyme immunoassay was shown to be more sensitive than electron microscopy and, therefore, 100% specific.

摘要

Rotazyme II是Rotazyme的一个较短版本(小于3小时),在229份人类粪便标本上,将其与电子显微镜检查以及Rotazyme在敏感性和特异性方面进行了比较。与电子显微镜检查相比,发现这种更新的检测方法敏感性为99.4%,特异性为97.3%,总体一致性为98.7%。通过封闭试验解决不一致结果后,Rotazyme II酶免疫测定法显示比电子显微镜检查更敏感,因此具有100%的特异性。

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