Chowdhury K, Garon C F, Israel M A
J Virol. 1983 Oct;48(1):40-51. doi: 10.1128/JVI.48.1.40-51.1983.
The nucleotide sequence and structural organization of integrated polyomavirus DNA and flanking host chromosomal DNA in a polyomavirus-induced hamster tumor cell line were evaluated. Although the proximal portion of the early transcriptional region was intact, the remainder of the integrated viral sequences was characterized by a series of rearrangements, including deletions, insertions, and a tandem duplication. Short direct repeats of different nucleotide sequences located on each side of the host-viral DNA junctions we examined were observed. Tumor cell chromosomal DNA flanking the integration site contained regions of patchy homology with viral DNA sequences which could have participated in the recombinational events leading to stable integration. The presence of several unusual structural features of tumor cell chromosomal DNA at the site of viral DNA integration, which were reminiscent of papovavirus DNA integrated into transformed cells, suggests that the recombinational mechanisms operative in vivo are accurately reflected by those functioning in vitro.
对多瘤病毒诱导的仓鼠肿瘤细胞系中整合的多瘤病毒DNA和侧翼宿主染色体DNA的核苷酸序列及结构组织进行了评估。虽然早期转录区域的近端部分是完整的，但整合病毒序列的其余部分具有一系列重排特征，包括缺失、插入和串联重复。我们观察到在我们检测的宿主-病毒DNA连接处两侧存在不同核苷酸序列的短直接重复序列。整合位点侧翼的肿瘤细胞染色体DNA包含与病毒DNA序列有局部同源性的区域，这些区域可能参与了导致稳定整合的重组事件。病毒DNA整合位点处肿瘤细胞染色体DNA的几个异常结构特征的存在，让人联想到整合到转化细胞中的乳头瘤多瘤空泡病毒科病毒DNA，这表明体内起作用的重组机制在体外的作用中得到了准确反映。
