Sato S, Makino M, Kawana R
Jpn J Med Sci Biol. 1983 Apr;36(2):85-95. doi: 10.7883/yoken1952.36.85.
By the use of serum-free Eagle's MEM instead of serum-supplemented culture medium in human embryo islet cell culture, it was possible to increase the proportion of beta cells identified by the indirect fluorescent antibody (IFA) technique. With the combination of the serum-deprived culture system and the double-label antibody technique, it was possible to show that the human embryo beta cells in culture were susceptible to infection with human papovavirus BK (BKV). Furthermore, this combination enabled us to assay quantitatively the BKV-infected beta cells, and it was shown that the proportion of BKV-infected beta cells in the islet cultures from three subjects were not significantly different either in BKV tumor antigen (T-Ag)-positive ratio or in BKV virion antigen (V-Ag)-positive ratio. The quantitative assay also showed that the percentages of BKV T-Ag- or V-Ag-positive cells in insulin-positive cells and in insulin-negative cells were almost the same.
在人胚胎胰岛细胞培养中，使用无血清的伊格尔氏最低必需培养基而非添加血清的培养基，能够提高通过间接荧光抗体（IFA）技术鉴定出的β细胞比例。通过血清剥夺培养系统与双标记抗体技术的结合，能够证明培养中的人胚胎β细胞易受人类乳头瘤病毒BK（BKV）感染。此外，这种结合使我们能够对受BKV感染的β细胞进行定量测定，结果表明，来自三名受试者的胰岛培养物中，受BKV感染的β细胞在BKV肿瘤抗原（T-Ag）阳性率或BKV病毒体抗原（V-Ag）阳性率方面并无显著差异。定量测定还显示，胰岛素阳性细胞和胰岛素阴性细胞中BKV T-Ag或V-Ag阳性细胞的百分比几乎相同。
