青霉素酶作为类固醇激素酶联免疫吸附测定中的标志物。

Penicillinase as a marker in enzyme-linked immunosorbent assays for steroid hormones.

作者信息

Joshi U M, Shah H P, Sankolli G M

出版信息

J Steroid Biochem. 1983 Jul;19(1B):419-21. doi: 10.1016/0022-4731(83)90197-8.

Abstract

Using penicillinase (E.C. 3.5.2.6) as marker and microtitre ELISA plates for immunoadsorption, sensitive and specific assays for two steroid hormones viz. testosterone in plasma and estrone-3-glucuronide in urine were developed. The entire assay can be performed in 4 h and requires simple laboratory equipment such as an incubator and a colorimeter. The enzyme labelled steroids have a shelf life of more than 3 years.

摘要

以青霉素酶（E.C. 3.5.2.6）为标记物，采用微量滴定酶联免疫吸附测定板进行免疫吸附，开发了两种甾体激素的灵敏且特异的检测方法，即血浆中的睾酮和尿液中的雌酮-3-葡萄糖醛酸苷。整个检测过程可在4小时内完成，所需实验室设备简单，如培养箱和比色计。酶标记的甾体激素保质期超过3年。