Nambara T, Ohkubo T, Yamaki T, Shimada K
J Steroid Biochem. 1984 Feb;20(2):621-6. doi: 10.1016/0022-4731(84)90133-x.
The preparation and antigenic properties of estrone-3-glucuronide- and estriol-3-glucuronide-bovine serum albumin conjugates in which the hapten is linked to the carrier protein through an (O-carboxymethyl)oxime bridge at the C-6 position on the steroid nucleus, have been described. Antibodies raised against the two immunogens in the rabbit possessed high specificity to estrone-3-glucuronide and estriol-3-glucuronide, respectively, exhibiting little cross-reactivities with other estrogen conjugates and no cross-reactions with related steroids except for free estrogens, their 3-methyl ethers and 3-sulfates. The cross-reactive antibodies were eliminated by partial immunoadsorption on affinity chromatographic media using the estrone-3-methyl ether 17-(O-carboxymethyl)oxime- and estriol-3-methyl ether 16 (or 17)-hemisuccinate-aminohexyl Sepharose conjugates, respectively. The purified antisera exhibited no cross-reactivities with free estrogens and ring A conjugates of estrone and estriol.
已描述了雌酮 -3- 葡萄糖醛酸苷和雌三醇 -3- 葡萄糖醛酸苷 - 牛血清白蛋白缀合物的制备及其抗原特性，其中半抗原通过甾体核 C-6 位的（O-羧甲基）肟桥与载体蛋白相连。在兔体内针对这两种免疫原产生的抗体分别对雌酮 -3- 葡萄糖醛酸苷和雌三醇 -3- 葡萄糖醛酸苷具有高度特异性，与其他雌激素缀合物几乎没有交叉反应，除了游离雌激素、它们的 3- 甲基醚和 3- 硫酸盐外，与相关甾体无交叉反应。分别使用雌酮 -3- 甲基醚 17-（O-羧甲基）肟和雌三醇 -3- 甲基醚 16（或 17）- 半琥珀酸 - 氨基己基琼脂糖缀合物在亲和色谱介质上进行部分免疫吸附，可消除交叉反应性抗体。纯化后的抗血清与游离雌激素以及雌酮和雌三醇的 A 环缀合物无交叉反应。
