Stromberg K, Benveniste R
Virology. 1983 Jul 30;128(2):518-23. doi: 10.1016/0042-6822(83)90281-7.
An examination of various rhesus monkey (Macaca mulatta) organs has shown a preference for type C viral antigen expression in the placenta. Separate cocultivations of isolated trophoblasts from 10 rhesus monkey placentas with cell lines from heterologous mammalian species led to rapid isolation of type C rhesus retrovirus in 4 of 10 cases. These four retrovirus isolates have been designated MMC-2 through MMC-5. Five of the remaining six sets of cocultivations grew simian foamy virus and were discontinued. Distinction of these viral isolates from the initial rhesus isolate (MMC-1) and the previous isolate from the stumptail monkey, Macaca arctoides (MAC-1), could be made by liquid DNA hybridization, although not by limited restriction endonuclease digestion. Both MAC-1 and MMC-1 were obtained in single long-term cocultivation experiments (over 7 months). The present isolates MMC-2 through MMC-5 were detected in 2 to 5 weeks. Consequently, primary trophoblast cells represent a useful differentiated cell type for isolation of infectious retrovirus from this primate species.
对多种恒河猴（猕猴）器官的检查显示，胎盘对C型病毒抗原表达具有偏好性。将来自10只恒河猴胎盘的分离滋养层细胞与异源哺乳动物细胞系进行单独共培养，在10例中有4例迅速分离出C型恒河猴逆转录病毒。这四种逆转录病毒分离株已被命名为MMC - 2至MMC - 5。其余六组共培养中有五组培养出了猴泡沫病毒，因此停止培养。这些病毒分离株与最初的恒河猴分离株（MMC - 1）以及之前从熊猴（食蟹猴）分离出的分离株（MAC - 1）的区分，可以通过液相DNA杂交来进行，尽管不能通过有限的限制性内切酶消化来区分。MAC - 1和MMC - 1都是在单次长期共培养实验（超过7个月）中获得的。目前的分离株MMC - 2至MMC - 5是在2至5周内检测到的。因此，原代滋养层细胞是从该灵长类动物物种中分离传染性逆转录病毒的一种有用的分化细胞类型。
