Gao M, Isom H C
J Virol. 1984 Nov;52(2):436-47. doi: 10.1128/JVI.52.2.436-447.1984.
Fragments of guinea pig cytomegalovirus (GPCMV) DNA produced by HindIII or EcoRI restriction endonuclease digestion were cloned into vectors pBR322 and pACYC184, and recombinant fragments representing ca. 97% of the genome were constructed. Hybridization of 32P-labeled cloned and gel-purified HindIII, EcoRI, and XbaI fragments to Southern blots of HindIII-, EcoRI-, and XbaI-cleaved GPCMV DNA verified the viral origin of cloned fragments and allowed construction of HindIII, EcoRI, and XbaI restriction maps. On the basis of the cloning and mapping experiments, the size of GPCMV DNA was calculated to include 239 kilobase pairs, corresponding to a molecular weight of 158 X 10(6). No cross-hybridization between any internal fragments was seen. We conclude that the GPCMV genome consists of a long unique sequence with terminal repeat sequences but without internal repeat regions. In addition, GPCMV DNA molecules exist in two forms. In the predominant form, the molecules demonstrate sequence homology between the terminal fragments; in the minor population, one terminal fragment is smaller by 0.7 X 10(6) daltons and is not homologous with the fragment at the other end of the physical map. The structural organization of GPCMV DNA is unique for a herpesvirus DNA, similar in its simplicity to the structure reported for murine cytomegalovirus DNA and quite dissimilar from that of human cytomegalovirus DNA.
用 HindIII 或 EcoRI 限制性内切酶消化产生的豚鼠巨细胞病毒（GPCMV）DNA 片段被克隆到载体 pBR322 和 pACYC184 中，并构建了代表约 97%基因组的重组片段。用 32P 标记的克隆且经凝胶纯化的 HindIII、EcoRI 和 XbaI 片段与 HindIII、EcoRI 和 XbaI 酶切的 GPCMV DNA 的 Southern 杂交印迹进行杂交，验证了克隆片段的病毒来源，并构建了 HindIII、EcoRI 和 XbaI 限制性图谱。基于克隆和图谱实验，计算出 GPCMV DNA 的大小为 239 千碱基对，对应分子量为 158×10⁶。未观察到任何内部片段之间的交叉杂交。我们得出结论，GPCMV 基因组由一个带有末端重复序列但无内部重复区域的长独特序列组成。此外
