Jarrell K F, Sprott G D
Arch Biochem Biophys. 1983 Aug;225(1):33-41. doi: 10.1016/0003-9861(83)90004-8.
The effects of numerous ionophores and inhibitors were tested on methane synthesis, intracellular ATP and potassium concentrations, and the proton motive force of the methanogenic archaebacterium Methanobacterium bryantii. M. bryantii had an internal pH near 6.8 (and hence little delta pH during growth) with an electrical potential of --127 mV in growth medium and --105 mV in a pH 6.5 buffer. The study has identified agents which, in M. bryantii, can effectively cause a decline of intracellular ATP (gramicidin, acetylene) and potassium concentrations (gramicidin, nigericin), inhibit methane synthesis (acetylene, gramicidin, nigericin, triphenylmethylphosphonium bromide), eliminate the electrical potential (high extracellular potassium ion concentrations), and dissipate artificially imposed, inside alkaline, pH gradients (monensin, nigericin, carbonyl cyanide m-chlorophenylhydrazone). Carbonyl cyanide m-chlorophenylhydrazone was generally ineffective in media or buffers reduced with cysteine-sulfide but could be effective in cysteine-free solutions reduced with hydrogen sulfide.
测试了多种离子载体和抑制剂对产甲烷古细菌布氏甲烷杆菌甲烷合成、细胞内ATP和钾离子浓度以及质子动力势的影响。布氏甲烷杆菌在生长培养基中的内部pH接近6.8（因此生长过程中ΔpH很小），其电势在生长培养基中为–127 mV，在pH 6.5缓冲液中为–105 mV。该研究确定了在布氏甲烷杆菌中能有效导致细胞内ATP浓度下降（短杆菌肽、乙炔）和钾离子浓度下降（短杆菌肽、尼日利亚菌素）、抑制甲烷合成（乙炔、短杆菌肽、尼日利亚菌素、溴化三苯甲基磷）、消除电势（高细胞外钾离子浓度）以及耗散人为施加的内部碱性pH梯度（莫能菌素、尼日利亚菌素、羰基氰化物间氯苯腙）的试剂。羰基氰化物间氯苯腙在用硫化物还原的培养基或缓冲液中通常无效，但在用硫化氢还原的无半胱氨酸溶液中可能有效。
