• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
A recording viscometer for assaying mammalian collagenase.一种用于测定哺乳动物胶原酶的记录式粘度计。
Biochem J. 1983 Jul 1;213(1):275-8. doi: 10.1042/bj2130275.
2
Action of bacterial collagenase on Ascaris cuticle collagen.
J Biochem. 1975 Nov;78(5):905-9. doi: 10.1093/oxfordjournals.jbchem.a130996.
3
Collagen treated with (+)-catechin becomes resistant to the action of mammalian collagenase.用（+）-儿茶素处理的胶原蛋白对哺乳动物胶原酶的作用产生抗性。
Experientia. 1981 Mar 15;37(3):221-3. doi: 10.1007/BF01991620.
4
Amperometric assay for collagenase. Amplification by the use of glucose oxidase conjugated to insoluble collagen.胶原酶的安培测定法。通过使用与不溶性胶原偶联的葡萄糖氧化酶进行放大。
Appl Biochem Biotechnol. 1983 Oct;8(5):407-14. doi: 10.1007/BF02779913.
5
Human platelet collagenase.
J Clin Invest. 1974 Jun;53(6):1647-54. doi: 10.1172/JCI107715.
6
A specific collagenase from rabbit fibroblasts in monolayer culture.来自单层培养兔成纤维细胞的一种特异性胶原酶。
Biochem J. 1974 Feb;137(2):373-85. doi: 10.1042/bj1370373.
7
Activation of latent collagenase of human leukocytes and gingival fluid by bacterial plaque.
J Dent Res. 1978 Jul-Aug;57(7-8):844-51. doi: 10.1177/00220345780570071401.
8
Insoluble collagen I. The effect of substrate weight on the rate of solubilisation of polymeric collagen fibrils by bacterial collagenase.不溶性I型胶原蛋白。底物重量对细菌胶原酶溶解聚合胶原纤维速率的影响。
Connect Tissue Res. 1975;4(1):1-6. doi: 10.3109/03008207509152190.
9
Observations on the different substrate behavior of tropocollagen molecules in solution and intermolecularly cross-linked tropocollagen within insoluble polymeric collagen fibrils.关于溶液中原胶原蛋白分子与不溶性聚合胶原纤维内分子间交联原胶原蛋白的不同底物行为的观察。
Biochem J. 1976 May 1;155(2):391-400. doi: 10.1042/bj1550391.
10
Purification, characterization and inhibition of human skin collagenase.人皮肤胶原酶的纯化、特性鉴定及抑制作用
Biochem J. 1978 Feb 1;169(2):265-76. doi: 10.1042/bj1690265.

引用本文的文献

1
Competitive inhibition of human skin collagenase by N-benzyloxycarbonyl-L-prolyl-L-alanyl-3-amino-2-oxopropyl-L-leucyl-L- alanylglycine ethyl ester.N-苄氧羰基-L-脯氨酰-L-丙氨酰-3-氨基-2-氧代丙基-L-亮氨酰-L-丙氨酰甘氨酸乙酯对人皮肤胶原酶的竞争性抑制作用
Biochem J. 1986 Nov 1;239(3):797-9. doi: 10.1042/bj2390797.

本文引用的文献

1
EXTRACTION OF COLLAGEN FROM CONNECTIVE TISSUE BY NEUTRAL SALT SOLUTIONS.用中性盐溶液从结缔组织中提取胶原蛋白。
Proc Natl Acad Sci U S A. 1955 Jan 15;41(1):1-7. doi: 10.1073/pnas.41.1.1.
2
An improved viscosimetric assay for vertebrate collagenase activity.一种用于检测脊椎动物胶原酶活性的改良粘度测定法。
Physiol Chem Phys. 1980;12(1):77-9.
3
Human skin collagenase. Isolation and mechanism of attack on the collagen molecule.人皮肤胶原酶。分离及其对胶原分子的攻击机制。
Biochim Biophys Acta. 1968 Mar 25;151(3):637-45. doi: 10.1016/0005-2744(68)90010-7.
4
The determination of collagen and elastin.胶原蛋白和弹性蛋白的测定
Methods Biochem Anal. 1967;15:25-76. doi: 10.1002/9780470110331.ch2.
5
A fluorescent assay for proteolytic enzymes.一种用于蛋白水解酶的荧光测定法。
Anal Biochem. 1973 Jun;53(2):484-90. doi: 10.1016/0003-2697(73)90098-5.
6
Human collagenase: identification and characterization of an enzyme from rheumatoid synovium in culture.人胶原酶：培养的类风湿性滑膜中一种酶的鉴定与特性研究
Science. 1967 Oct 27;158(3800):499-502. doi: 10.1126/science.158.3800.499.
7
Flow-through viscometer for use in the automated determination of hydrolytic enzyme activities: application in protease, amylase, and pectinase assays.
Anal Biochem. 1978 Oct 1;90(1):192-203. doi: 10.1016/0003-2697(78)90023-4.
8
Purification, characterization and inhibition of human skin collagenase.人皮肤胶原酶的纯化、特性鉴定及抑制作用
Biochem J. 1978 Feb 1;169(2):265-76. doi: 10.1042/bj1690265.
9
Collagenase production by human skin fibroblasts.人皮肤成纤维细胞产生胶原酶的情况。
Biochem Biophys Res Commun. 1975 May 5;64(1):232-40. doi: 10.1016/0006-291x(75)90243-0.
10
Purification and characterization of a collagenase extracted from rabbit tumours.从兔肿瘤中提取的胶原酶的纯化与特性分析
Biochem J. 1975 Oct;152(1):131-42. doi: 10.1042/bj1520131.

一种用于测定哺乳动物胶原酶的记录式粘度计。

A recording viscometer for assaying mammalian collagenase.

作者信息

Macartney H W, Bates S R, Blumson N L, Nelson D, Jamison D, Elmore D T

出版信息

Biochem J. 1983 Jul 1;213(1):275-8. doi: 10.1042/bj2130275.

DOI:10.1042/bj2130275
PMID:6311160
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1152120/
Abstract

A recording viscometer for monitoring the action of mammalian collagenase on soluble collagen is described. For this system, where only one peptide bond is cleaved per subunit, it is shown theoretically that the decrease in viscosity is proportional to the fraction of molecules cleaved. Experimental confirmation was obtained by parallel monitoring of hydrolysis by using the fluorescamine assay of liberated amino groups. The initial velocity of reaction is proportional to substrate concentration and enzyme concentration.

摘要

描述了一种用于监测哺乳动物胶原酶对可溶性胶原作用的记录式粘度计。对于该系统，每个亚基仅一个肽键被裂解，理论表明粘度降低与裂解分子的比例成正比。通过使用游离氨基的荧光胺测定法并行监测水解获得了实验证实。反应的初始速度与底物浓度和酶浓度成正比。