一种用于测定哺乳动物胶原酶的记录式粘度计。
A recording viscometer for assaying mammalian collagenase.
作者信息
Macartney H W, Bates S R, Blumson N L, Nelson D, Jamison D, Elmore D T
出版信息
Biochem J. 1983 Jul 1;213(1):275-8. doi: 10.1042/bj2130275.
Abstract
A recording viscometer for monitoring the action of mammalian collagenase on soluble collagen is described. For this system, where only one peptide bond is cleaved per subunit, it is shown theoretically that the decrease in viscosity is proportional to the fraction of molecules cleaved. Experimental confirmation was obtained by parallel monitoring of hydrolysis by using the fluorescamine assay of liberated amino groups. The initial velocity of reaction is proportional to substrate concentration and enzyme concentration.
摘要
描述了一种用于监测哺乳动物胶原酶对可溶性胶原作用的记录式粘度计。对于该系统，每个亚基仅一个肽键被裂解，理论表明粘度降低与裂解分子的比例成正比。通过使用游离氨基的荧光胺测定法并行监测水解获得了实验证实。反应的初始速度与底物浓度和酶浓度成正比。
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