Chevillard C, Saavedra J M
Brain Res. 1983 Aug 8;272(2):283-90. doi: 10.1016/0006-8993(83)90575-9.
We studied the activity of angiotensin I converting enzyme (ACE, kininase II, E.C. 3.4.15.1) in discrete areas of the brainstem and limbic system, and in circumventricular organs, pineal gland and choroid plexus of homozygous Brattleboro rats (DI) which are characterized by vasopressin deficiency and diabetes insipidus, with or without vasopressin replacement. We also determined ACE activity in heterozygous Brattleboro (HZ) and Long-Evans (LE) control rats. We found changes in ACE activity in several brain areas and the pineal gland of Brattleboro rats. ACE activity was increased in DI rats with respect to HZ and LE controls in the A1 area of the brainstem, locus coeruleus, and triangular nucleus of the septum. ACE activity in the A2 area of the brainstem, the nucleus tractus spinalis nervi trigeminii and the pineal gland was enhanced in both HZ and DI rats with respect to that of LE controls, but was not different between HZ and DI rats. ACE activity did not change in the other extrahypothalamic areas studied. The elevated ACE activity in extrahypothalamic areas of DI rats was not reversed by vasopressin replacement. These results suggest that a relationship between central vasopressin and angiotensin or bradykinin systems may exist in selective extrahypothalamic areas of the rat brain, and that peripherally administered vasopressin cannot influence this relationship.
我们研究了纯合布拉特洛伯勒大鼠（DI）脑干和边缘系统的离散区域、室周器官、松果体和脉络丛中血管紧张素I转换酶（ACE，激肽酶II，E.C. 3.4.15.1）的活性，这些大鼠的特征是抗利尿激素缺乏和尿崩症，无论是否进行抗利尿激素替代治疗。我们还测定了杂合布拉特洛伯勒大鼠（HZ）和朗-埃文斯大鼠（LE）作为对照大鼠的ACE活性。我们发现布拉特洛伯勒大鼠的几个脑区和松果体中的ACE活性发生了变化。与HZ和LE对照相比，DI大鼠脑干的A1区、蓝斑和隔三角核中的ACE活性增加。与LE对照相比，HZ和DI大鼠脑干的A2区、三叉神经脊束核和松果体中的ACE活性均增强，但HZ和DI大鼠之间没有差异。在所研究的其他下丘脑外区域，ACE活性没有变化。DI大鼠下丘脑外区域升高的ACE活性不会因抗利尿激素替代治疗而逆转。这些结果表明，在大鼠脑的选择性下丘脑外区域中，中枢抗利尿激素与血管紧张素或缓激肽系统之间可能存在某种关系，并且外周给予的抗利尿激素不能影响这种关系。
