Iwasaki H, Kikuchi M, Ohtsuki I, Enjoji M, Suenaga N, Mori R
Cancer. 1983 Nov 1;52(9):1653-61. doi: 10.1002/1097-0142(19831101)52:9<1653::aid-cncr2820520918>3.0.co;2-c.
Cell cultures were carried out from four patients with infantile digital fibromatosis. The cultured cells, which contained cytoplasmic inclusions identical to those of the original tumor cells, possessed cortical bundles of microfilaments, rich network of granular endoplasmic reticulum, and well-developed Golgi complex. To demonstrate the distribution of actin filaments in the cultured cells, the heavy meromyosin-binding method was applied to saponin-treated cells from one of the four patients. The microfilaments constituting the inclusions as well as the cortical bundles were decorated with heavy meromyosin, presenting the "arrowhead complexes" specific for actin filaments. The inclusion may represent abnormal contraction of actin filaments in the cytoplasm of myofibroblasts.
对4例婴儿指纤维瘤病患者进行了细胞培养。培养的细胞含有与原始肿瘤细胞相同的胞质内含物，具有微丝的皮质束、丰富的颗粒内质网网络和发达的高尔基体复合体。为了证明培养细胞中肌动蛋白丝的分布，对4例患者之一经皂素处理的细胞应用了重酶解肌球蛋白结合法。构成内含物以及皮质束的微丝被重酶解肌球蛋白修饰，呈现出肌动蛋白丝特有的“箭头复合体”。这种内含物可能代表肌成纤维细胞胞质中肌动蛋白丝的异常收缩。
