Lössner B, Rose S P
J Neurochem. 1983 Nov;41(5):1357-63. doi: 10.1111/j.1471-4159.1983.tb00833.x.
Fucokinase (EC 2.7.1.52) activity was estimated in supernatants of homogenate from day-old chick forebrain. Enzyme kinetic studies gave a Km of 4.5 X 10(-6) M and Vmax of 3.72 nmol fucose converted into fucose-1-phosphate/mg prot/h. The pH optimum was 7.5. The enzyme is thus considerably more active than was reported for other species and tissues. There were no differences in enzyme activity between the four forebrain regions studied. One hour after chicks were trained on a one-trial passive avoidance learning paradigm, enzyme activity in the right forebrain base increased 14% over control values (p less than 0.02). The 11.3% increase in activity in the left forebrain base and 10.3% increase in the left roof were not statistically significant. The relationship of this change to the increased fucose incorporation into glycoproteins known to occur over a similar time period and the significance of the lateralization of the increase are discussed.
在一日龄雏鸡前脑匀浆的上清液中测定了岩藻糖激酶（EC 2.7.1.52）的活性。酶动力学研究得出米氏常数（Km）为4.5×10⁻⁶M，最大反应速度（Vmax）为3.72纳摩尔岩藻糖转化为岩藻糖-1-磷酸/毫克蛋白/小时。最适pH值为7.5。因此，该酶的活性比其他物种和组织所报道的活性要高得多。在所研究的四个前脑区域之间，酶活性没有差异。在雏鸡接受单次被动回避学习范式训练一小时后，右前脑基部的酶活性比对照值增加了14%（p<0.02）。左前脑基部活性增加11.3%以及左顶叶活性增加10.3%均无统计学意义。本文讨论了这种变化与已知在相似时间段内发生的岩藻糖掺入糖蛋白增加之间的关系，以及增加的侧化现象的意义。
