Holland C A, Wozney J, Hopkins N
J Virol. 1983 Sep;47(3):413-20. doi: 10.1128/JVI.47.3.413-420.1983.
We determined the nucleotide sequence and predicted the amino acid sequence of the gp70 gene of MCF 247, a recombinant murine retrovirus isolated from an AKR mouse. Information specifying the first 286 amino acids of the protein was probably derived from the presumptive nonecotropic parent of MCF 247, whereas the C-terminal 154 amino acids were probably derived from the ecotropic parent Akv. The nonecotropic sequences at the amino terminus of MCF 247 show only 38% homology, at the amino acid level, to those of Akv. In contrast, these sequences are strikingly similar (99% homologous) to those reported for another MCF virus. Moloney MCF, which was isolated from a BALB/c mouse. Moloney MCF also has ecotropic-derived sequences encoding the C-terminal portion of its gp70 protein; however, the recombination event that introduced these sequences occurs 213 nucleotides further towards the C terminus of gp70 than it does in MCF 247.
我们测定了MCF 247（一种从AKR小鼠中分离出的重组鼠逆转录病毒）的gp70基因的核苷酸序列，并预测了其氨基酸序列。指定该蛋白质前286个氨基酸的信息可能源自MCF 247假定的非亲嗜性亲本，而C末端的154个氨基酸可能源自亲嗜性亲本Akv。MCF 247氨基末端的非亲嗜性序列在氨基酸水平上与Akv的序列仅显示38%的同源性。相比之下，这些序列与另一种MCF病毒（从BALB/c小鼠中分离出的莫洛尼MCF）报道的序列惊人地相似（99%同源）。莫洛尼MCF也有亲嗜性来源的序列，编码其gp70蛋白的C末端部分；然而，引入这些序列的重组事件发生在gp70的C末端比MCF 247中更远213个核苷酸处。
