Schultz A, Rein A, Henderson L, Oroszlan S
J Virol. 1983 Mar;45(3):995-1003. doi: 10.1128/JVI.45.3.995-1003.1983.
Two murine leukemia viruses were isolated from JLS-V9 cells which had been infected with Rauscher plasma virus. One virus was XC positive and failed to grow on mink or cat cells and thus was an ecotropic virus. The other virus formed cytopathic foci on mink cells, was XC negative, and fell into the mink cell focus-forming (MCF) viral interference group and was thus an MCF virus. The glycoproteins of the two viruses could be distinguished immunologically, by peptide mapping, and by size in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The MCF virus produced gp69, and the ecotropic virus produced gp71, explaining the origin of the heterogeneous glycoprotein (gp69 and gp71) of Rauscher leukemia virus. Amino-terminal sequences of gp69 and gp71 were determined. The MCF sequence was distinct from the ecotropic sequence, but retained partial homology to it. The data show that the glycoproteins are encoded by related yet distinct genes. The protein structural data support the proposal that MCF virus gp70 molecules have nonecotropic sequences at the amino terminus, with ecotropic sequences occurring at the 3' end of the gene. The Rauscher MCF virus glycoprotein lacks a glycosylation site found at position 12 of the ecotropic sequence.
从感染了劳舍尔血浆病毒的JLS-V9细胞中分离出两种鼠白血病病毒。一种病毒为XC阳性，不能在貂或猫细胞上生长，因此是嗜亲性病毒。另一种病毒在貂细胞上形成细胞病变灶，为XC阴性，属于貂细胞灶形成（MCF）病毒干扰组，因此是一种MCF病毒。这两种病毒的糖蛋白可以通过免疫、肽图谱分析以及在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中的大小来区分。MCF病毒产生gp69，嗜亲性病毒产生gp71，这解释了劳舍尔白血病病毒异源糖蛋白（gp69和gp71）的来源。测定了gp69和gp71的氨基末端序列。MCF序列与嗜亲性序列不同，但与之保留部分同源性。数据表明，这些糖蛋白由相关但不同的基因编码。蛋白质结构数据支持以下提议：MCF病毒gp70分子在氨基末端具有非嗜亲性序列，嗜亲性序列出现在基因的3'端。劳舍尔MCF病毒糖蛋白在嗜亲性序列第12位处缺少一个糖基化位点。
