Simultaneous staining of proteins during polyacrylamide gel electrophoresis in acidic gels by countermigration of Coomassie brilliant blue R-250.
作者信息
Varghese G, Diwan A M
出版信息
Anal Biochem. 1983 Jul 15;132(2):481-3. doi: 10.1016/0003-2697(83)90037-4.
PMID:6312843
Abstract
A method for the simultaneous staining of proteins during polyacrylamide gel electrophoresis with Coomassie brilliant blue R-250 at pH 2.5 is described. Calf thymus whole histone and cytochrome c were stained by this method and the results obtained were similar to that obtained by staining after electrophoresis.
摘要
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