生物酚类抗氧化剂与细胞色素P-450模型系统产生的超氧化物的反应。

Reaction of biological phenolic antioxidants with superoxide generated by cytochrome P-450 model system.

作者信息

Tajima K, Sakamoto M, Okada K, Mukai K, Ishizu K, Sakurai H, Mori H

出版信息

Biochem Biophys Res Commun. 1983 Sep 30;115(3):1002-8. doi: 10.1016/s0006-291x(83)80034-5.

Abstract

Scavenging of a superoxide and simultaneous formation of free radicals with phenolic antioxidants were investigated with cobalt and iron tetraphenylporphyrin-thiolate complexes as models of P-450 enzymes. The kinetics of decay of the superoxide and development of free radical ESR signal intensities were studied. Based on a molecular orbital calculation of the hyperfine splitting, the radical species generated were confirmed to be those of phenoxyl radicals. The biological implication of superoxide, including active oxygen forms, for the reaction was discussed.

摘要

以钴和铁四苯基卟啉硫醇盐配合物作为P-450酶的模型，研究了超氧化物的清除以及酚类抗氧化剂同时形成自由基的过程。研究了超氧化物衰减动力学和自由基ESR信号强度的变化。基于超精细分裂的分子轨道计算，确认生成的自由基物种为苯氧基自由基。讨论了包括活性氧形式在内的超氧化物对该反应的生物学意义。