Chai T, Wu V, Foulds J
J Bacteriol. 1982 Aug;151(2):983-8. doi: 10.1128/jb.151.2.983-988.1982.
ompF cells were completely resistant to colicin A, whereas btuB cells were partially resistant. The OmpF protein, in the presence of added lipopolysaccharide, inactivated colicin A. This inactivation was enhanced by added btuB gene product, btuB gene product with lipopolysaccharide did not inactivate colicin A. These data, together with the observation that vitamin B12 protected btuB+ cells from the killing effect of colicin A, suggest that the colicin A receptor in Escherichia coli K-12 is composed of the OmpF protein, the btuB gene product, and lipopolysaccharide.
ompF细胞对大肠菌素A完全耐药,而btuB细胞则部分耐药。在添加脂多糖的情况下,OmpF蛋白可使大肠菌素A失活。添加的btuB基因产物可增强这种失活作用,btuB基因产物与脂多糖一起则不会使大肠菌素A失活。这些数据,连同维生素B12可保护btuB+细胞免受大肠菌素A杀伤作用的观察结果,表明大肠杆菌K-12中的大肠菌素A受体由OmpF蛋白、btuB基因产物和脂多糖组成。