Peisach J, Beinert H, Emptage M H, Mims W B, Fee J A, Orme-Johnson W H, Rendina A R, Orme-Johnson N R
J Biol Chem. 1983 Nov 10;258(21):13014-6.
We describe a method for the differentiation of 3-iron from 2-iron and 4-iron Fe/S proteins based on consideration of both the magnetic field dependence of shifts in g induced by an externally applied electric field (LEFE) and the continuous wave EPR spectra properties. The magnetic field dependence and the magnitude of the LEFE for 3-iron ferredoxins are similar to those for 4-iron ferredoxins but differ considerably from those for 2-iron ferredoxins or for high potential iron proteins. Furthermore, as 3-iron ferredoxins and high potential iron proteins are EPR-active when oxidized while 2-iron and 4-iron ferredoxins are only EPR-active when reduced, the differentiation among all of them can be made on the basis of both continuous wave EPR and LEFE properties, but not by each individually.
我们描述了一种基于对外加电场诱导的g位移的磁场依赖性（LEFE）和连续波EPR光谱特性，区分三铁与二铁和四铁铁硫蛋白的方法。三铁铁氧化还原蛋白的LEFE的磁场依赖性和大小与四铁铁氧化还原蛋白相似，但与二铁铁氧化还原蛋白或高电位铁蛋白的LEFE有很大差异。此外，由于三铁铁氧化还原蛋白和高电位铁蛋白在氧化时具有EPR活性，而二铁和四铁铁氧化还原蛋白仅在还原时具有EPR活性，因此可以根据连续波EPR和LEFE特性对它们进行区分，但不能单独依据其中任何一项。
