Physiologie Cellulaire Végétale (U.A.-C.N.R.S. 576), DRF/PCV Centre D'Etudes Nucleaires et Universite Scientifique et Medicale, 85X F38041 Grenoble Cedex, France.
Plant Physiol. 1986 May;81(1):247-52. doi: 10.1104/pp.81.1.247.
Electron paramagnetic resonance (EPR) characteristics of the iron-sulfur clusters of potato tuber mitochondria have been examined in various subfractions of the mitochondria. We confirm that EPR signals comparable to those of the iron-sulfur proteins of mammalian mitochondria respiratory complexes are also present in plant mitochondria. Two distinct iron-sulfur centers paramagnetic in the oxidized state exhibit signals which differ in their detailed line shape and field position. One of these which is present in the inner membrane corresponds to center S.3. The EPR spectrum of the soluble fraction revealed the presence of another center with a low field maximum at g = 2.03 and is associated with aconitase. The EPR signal observed in the mitochondrial matrix from potato tuber and characteristic of 3Fe cluster is significantly changed in shape after addition of citrate and differs clearly from the spectrum of pig heart mitochondrial aconitase. The aconitase in plant mitochondria differs from that of mammalian mitochondria by several features.
已在各种亚线粒体部分中检查了块茎线粒体的铁硫簇的电子顺磁共振（EPR）特征。我们确认，植物线粒体中也存在与哺乳动物线粒体呼吸复合物的铁硫蛋白相当的 EPR 信号。两种在氧化状态下具有顺磁性的不同铁硫中心表现出在其详细线形状和场位置上有所不同的信号。其中一个存在于内膜中，对应于中心 S.3。可溶性部分的 EPR 光谱揭示了另一个中心的存在，其低场最大值在 g = 2.03 处，并与 aconitase 相关。从块茎的线粒体基质中观察到的、具有 3Fe 簇特征的 EPR 信号在添加柠檬酸盐后形状明显改变，并且与猪心线粒体 aconitase 的光谱明显不同。植物线粒体中的 aconitase 与哺乳动物线粒体中的 aconitase 有几个不同的特征。
