Weigle K A, Grose C
J Infect Dis. 1983 Oct;148(4):630-8. doi: 10.1093/infdis/148.4.630.
Human cells infected with varicella-zoster virus (VZV) produce at least three major virus-specific, immunogenic glycoproteins: gp118, gp98, and gp62. Since glycoproteins gp98 and gp62 were found to be prominent constituents of the infected cell membrane, a murine monoclonal antibody (clone 3B3) that reacted avidly with this glycoprotein complex was selected as a probe for detection of VZV replication in laboratory and clinical settings. Cultured cells of human, simian, and caviid origin, when infected with wild-type isolates as well as laboratory and vaccine strains of VZV all expressed these viral glycoproteins. The monoclonal antibody immunostained the basal and malpighian epithelial layers of a zoster vesicle biopsy specimen and also reacted with all specimens of vesicular cells obtained from epidemiologically unrelated patients with chickenpox and zoster. Thus, these studies demonstrate that the VZV-specific glycoprotein complex gp98/gp62 is highly conserved, abundantly expressed, and easily detected with a monoclonal antibody probe.
感染水痘-带状疱疹病毒（VZV）的人类细胞会产生至少三种主要的病毒特异性免疫原性糖蛋白：gp118、gp98和gp62。由于发现糖蛋白gp98和gp62是受感染细胞膜的主要成分，因此选择一种与该糖蛋白复合物强烈反应的鼠单克隆抗体（克隆3B3）作为在实验室和临床环境中检测VZV复制的探针。人、猴和豚鼠来源的培养细胞，在感染野生型分离株以及VZV的实验室和疫苗株时，均表达这些病毒糖蛋白。该单克隆抗体对带状疱疹水疱活检标本的基底和马尔皮基上皮层进行免疫染色，并且还与从流行病学上不相关的水痘和带状疱疹患者获得的所有水疱细胞标本发生反应。因此，这些研究表明，VZV特异性糖蛋白复合物gp98/gp62高度保守、大量表达，并且易于用单克隆抗体探针检测。
