Nagai Y, Yoshida T, Hamaguchi M, Nagura H, Hasegawa H, Yoshimura S, Watanabe K
Microbiol Immunol. 1983;27(6):531-45. doi: 10.1111/j.1348-0421.1983.tb00614.x.
The major structural proteins of Newcastle disease virus and Sendai virus were localized in infected BHK-21 and MDBK cells by ultrastructural immunoperoxidase cytochemistry using antibodies against the individual viral protein antigens. The intracellular glycoproteins were strictly membrane bound, being localized in the rough endoplasmic reticulum (RER), perinuclear spaces, smooth membrane vesicles, and presumed Golgi apparatus. The nucleocapsid proteins were detected exclusively in membrane free cytosol and accumulated there, forming inclusions. The membrane (M) protein was found both in cytosol and on RER. The viral proteins on RER exhibited a distinct site specificity; the glycoproteins were facing the lumen of RER whereas M protein was present at the outer cytoplasmic surface. All the viral proteins were detectable at the plasma membrane where virus assembly takes place. However, their modes of distribution differed remarkably. The glycoproteins were spread widely over the entire cell surface including the areas of virus budding and those of normal morphology, whereas M protein was localized in restricted areas of the membrane, frequently forming a patch of virus specific membrane. The presence of nucleocapsids was confined to the virus particles budding from the plasma membrane. These results complement and extend the earlier morphological and biochemical data on the assembly or morphogenesis of paramyxoviruses.
利用针对个别病毒蛋白抗原的抗体，通过超微结构免疫过氧化物酶细胞化学技术，对新城疫病毒和仙台病毒的主要结构蛋白在感染的BHK - 21细胞和MDBK细胞中的定位进行了研究。细胞内的糖蛋白严格与膜结合，定位于粗面内质网（RER）、核周间隙、光滑膜泡以及推测的高尔基体中。核衣壳蛋白仅在无膜的胞质溶胶中被检测到，并在那里积累形成包涵体。膜（M）蛋白在胞质溶胶和粗面内质网上均有发现。粗面内质网上的病毒蛋白表现出明显的位点特异性；糖蛋白面向粗面内质网腔，而M蛋白则位于细胞质外表面。在病毒装配发生的质膜上可检测到所有病毒蛋白。然而，它们的分布模式有显著差异。糖蛋白广泛分布于整个细胞表面，包括病毒出芽区域和正常形态区域，而M蛋白则定位于膜的受限区域，常常形成病毒特异性膜斑。核衣壳仅存在于从质膜出芽的病毒颗粒中。这些结果补充并扩展了关于副粘病毒装配或形态发生的早期形态学和生化数据。
