Seto J T, Wahn K, Becht H
Arch Virol. 1980;65(3-4):247-55. doi: 10.1007/BF01314541.
Chorioallantoic membrane (CAM) tissue cultures were found to be permissive for representative paramyxoviruses. The CAM cells can be used for plaque assay without the presence of trypsin. Ultrastructures of CAM cells infected with paramyxovirus Yucaipa (PMY), Sendai virus, and NDV were different. Nucleocapsids were readily seen in budding structures of cells infected with PMY, and in Sendai virus infected cells, large clusters of nucleocapsids were clearly evident in the cytoplasm. The site of glycoprotein cleavage does not have any effect on the nature of budding. It appears that a factor or factors in addition to the nature of the plasma membrane influences the morphology of cells infected with paramyxoviruses.
发现绒毛尿囊膜(CAM)组织培养物对代表性副粘病毒具有易感性。CAM细胞可用于空斑测定,无需胰蛋白酶。感染副粘病毒尤凯帕株(PMY)、仙台病毒和新城疫病毒(NDV)的CAM细胞的超微结构有所不同。在感染PMY的细胞出芽结构中很容易看到核衣壳,而在感染仙台病毒的细胞中,细胞质中明显可见大量核衣壳簇。糖蛋白切割位点对出芽性质没有任何影响。看来,除质膜性质外的一个或多个因素会影响感染副粘病毒的细胞形态。