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对感染代表性副粘病毒的绒毛尿囊膜培养细胞进行的电子显微镜研究。

Electron microscope study of cultured cells of the chorioallantoic membrane infected with representative paramyxoviruses.

作者信息

Seto J T, Wahn K, Becht H

出版信息

Arch Virol. 1980;65(3-4):247-55. doi: 10.1007/BF01314541.

DOI:10.1007/BF01314541
PMID:6251785
Abstract

Chorioallantoic membrane (CAM) tissue cultures were found to be permissive for representative paramyxoviruses. The CAM cells can be used for plaque assay without the presence of trypsin. Ultrastructures of CAM cells infected with paramyxovirus Yucaipa (PMY), Sendai virus, and NDV were different. Nucleocapsids were readily seen in budding structures of cells infected with PMY, and in Sendai virus infected cells, large clusters of nucleocapsids were clearly evident in the cytoplasm. The site of glycoprotein cleavage does not have any effect on the nature of budding. It appears that a factor or factors in addition to the nature of the plasma membrane influences the morphology of cells infected with paramyxoviruses.

摘要

发现绒毛尿囊膜(CAM)组织培养物对代表性副粘病毒具有易感性。CAM细胞可用于空斑测定,无需胰蛋白酶。感染副粘病毒尤凯帕株(PMY)、仙台病毒和新城疫病毒(NDV)的CAM细胞的超微结构有所不同。在感染PMY的细胞出芽结构中很容易看到核衣壳,而在感染仙台病毒的细胞中,细胞质中明显可见大量核衣壳簇。糖蛋白切割位点对出芽性质没有任何影响。看来,除质膜性质外的一个或多个因素会影响感染副粘病毒的细胞形态。

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1
Electron microscope study of cultured cells of the chorioallantoic membrane infected with representative paramyxoviruses.对感染代表性副粘病毒的绒毛尿囊膜培养细胞进行的电子显微镜研究。
Arch Virol. 1980;65(3-4):247-55. doi: 10.1007/BF01314541.
2
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引用本文的文献

1
The site of cleavage in infected cells and polypeptides of representative paramyxoviruses grown in cultured cells of the chorioallantoic membrane.感染细胞中的裂解位点以及在绒毛尿囊膜培养细胞中生长的代表性副粘病毒的多肽。
Arch Virol. 1981;67(1):19-30. doi: 10.1007/BF01314598.
2
Persistent infections with Sendai virus and Newcastle disease viruses.仙台病毒和新城疫病毒的持续性感染。
Arch Virol. 1986;89(1-4):225-33. doi: 10.1007/BF01309891.

本文引用的文献

1
Functional significance of sialidose during influenza virus multiplication.流感病毒增殖过程中唾液酸酶的功能意义。
Virology. 1966 Dec;30(4):731-7. doi: 10.1016/0042-6822(66)90178-4.
2
Newcastle disease virus infection of L cells.新城疫病毒对L细胞的感染。
J Virol. 1974 Jul;14(1):162-9. doi: 10.1128/JVI.14.1.162-169.1974.
3
Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity of proteolytic cleavage of an inactive precursor protein of Sendai virus.
副粘病毒糖蛋白生物活性的鉴定。仙台病毒无活性前体蛋白经蛋白水解切割后细胞融合、溶血及感染性的激活。
Virology. 1974 Feb;57(2):475-90. doi: 10.1016/0042-6822(74)90187-1.
4
Trypsin action on the growth of Sendai virus in tissue culture cells. 3. Structural difference of Sendai viruses grown in eggs and tissue culture cells.胰蛋白酶对仙台病毒在组织培养细胞中生长的作用。3. 在鸡胚和组织培养细胞中生长的仙台病毒的结构差异。
J Virol. 1973 Dec;12(6):1457-65. doi: 10.1128/JVI.12.6.1457-1465.1973.
5
Morphogenesis of type 2 parainfluenza virus examined by light and electron microscopy.通过光学显微镜和电子显微镜检查2型副流感病毒的形态发生。
J Virol. 1967 Feb;1(1):215-37. doi: 10.1128/JVI.1.1.215-237.1967.
6
An electron microscopic study of moderate and virulent virus-cell interactions of the parainfluenza virus SV5.副流感病毒SV5中度和强毒株与细胞相互作用的电子显微镜研究
Virology. 1966 Nov;30(3):411-26. doi: 10.1016/0042-6822(66)90119-x.
7
Studies on the assembly of the envelope of Newcastle disease virus.新城疫病毒包膜组装的研究。
Virology. 1976 Feb;69(2):523-38. doi: 10.1016/0042-6822(76)90482-7.
8
Ortho- and paramyxoviruses from migrating feral ducks: characterization of a new group of influenza A viruses.迁徙野鸭中的正粘病毒和副粘病毒:一组新型甲型流感病毒的特征
J Gen Virol. 1976 Aug;32(2):217-25. doi: 10.1099/0022-1317-32-2-217.
9
Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus.病毒糖蛋白的蛋白水解切割及其对新城疫病毒毒力的意义。
Virology. 1976 Jul 15;72(2):494-508. doi: 10.1016/0042-6822(76)90178-1.
10
The spread of a pathogenic and an apathogenic strain of Newcastle disease virus in the chick embryo as depending on the protease sensitivity of the virus glycoproteins.新城疫病毒致病性和非致病性毒株在鸡胚中的传播与病毒糖蛋白对蛋白酶敏感性的关系
J Gen Virol. 1979 Nov;45(2):263-72. doi: 10.1099/0022-1317-45-2-263.