枯草芽孢杆菌核糖体RNA操纵子rrnB的核苷酸序列

Nucleotide sequence of the Bacillus subtilis ribosomal RNA operon, rrnB.

作者信息

Green C J, Stewart G C, Hollis M A, Vold B S, Bott K F

出版信息

Gene. 1985;37(1-3):261-6. doi: 10.1016/0378-1119(85)90281-1.

Abstract

The primary sequence of DNA covering a complete ribosomal RNA (rRNA) operon from Bacillus subtilis, designated rrnB has been elucidated. Following a set of tandem promoters, rrnB encodes: (i) a 16S and a 23S rRNA determinant with no tRNA spacer region in between; (ii) a 5S rRNA determinant; and (iii) 21 contiguous tRNA species; before (iv) two characteristic terminator hairpins are found. More than 7 kb are included within this operon, which maps between aroG and thr5 on the B. subtilis chromosome. This represents the first report of the entire sequence of an rRNA operon from B. subtilis or from any Gram-positive organism.

摘要

已阐明来自枯草芽孢杆菌的一个完整核糖体RNA（rRNA）操纵子（命名为rrnB）的DNA一级序列。在一组串联启动子之后，rrnB编码：（i）一个16S和一个23S rRNA决定簇，两者之间没有tRNA间隔区；（ii）一个5S rRNA决定簇；以及（iii）21个连续的tRNA种类；然后（iv）发现两个特征性终止发夹结构。该操纵子包含超过7 kb的序列，定位于枯草芽孢杆菌染色体上的aroG和thr5之间。这是关于枯草芽孢杆菌或任何革兰氏阳性菌rRNA操纵子全序列的首次报道。