Muhich M L, Simpson L
Nucleic Acids Res. 1986 Jul 11;14(13):5531-56. doi: 10.1093/nar/14.13.5531.
Multiple sequence classes of kinetoplast minicircle DNA from Leishmania tarentolae were cleaved by mung bean nuclease in the presence of formamide, yielding unit length linear molecules which retained the anomalous electrophoretic mobility in acrylamide characteristic of minicircle DNA. No specific cleavage site sequence common to all minicircle sequence classes was apparent, although the main region of nuclease cleavage was localized approximately 350 bp from the unique SmaI restriction site of the conserved region found in all minicircle sequence classes. Covalent closure of the minicircle substrate was not a requirement for cleavage, as linearized network-derived or cloned minicircles were also cleaved by mung bean nuclease at similar locations. The partial sequences of several new minicircle sequence classes released from the network by mung bean nuclease are also reported.
来自热带利什曼原虫的动质体小环DNA的多个序列类别在甲酰胺存在的情况下被绿豆核酸酶切割，产生单位长度的线性分子，这些分子在丙烯酰胺中保留了小环DNA特有的异常电泳迁移率。尽管核酸酶切割的主要区域位于所有小环序列类别中保守区域的独特SmaI限制性位点约350 bp处，但并未发现所有小环序列类别共有的特定切割位点序列。小环底物的共价闭合不是切割的必要条件，因为线性化的网络衍生或克隆小环也会被绿豆核酸酶在相似位置切割。还报道了通过绿豆核酸酶从网络中释放的几个新小环序列类别的部分序列。
