Mueser T, Isaac L, Radulovacki M
Physiol Behav. 1983 Aug;31(2):237-9. doi: 10.1016/0031-9384(83)90126-9.
Rats were placed on either small (subjected to REM sleep deprivation) or large circular platforms (stress controls) surrounded by water for 96 hours. Six animals from each group were either decapitated immediately following 96 hours or placed in cages and decapitated 6 hours later. Benzodiazepine receptor binding in rat brain cortices and brain stems was assessed using 3H-diazepam as the ligand. Scatchard analysis of these data showed that neither REM sleep deprivation nor REM sleep rebound affected the kinetics of 3H-diazepam binding (Bmax or apparent KD) in vitro.
将大鼠置于被水环绕的小圆形平台（进行快速眼动睡眠剥夺）或大圆形平台（应激对照组）上96小时。每组6只动物在96小时结束后立即断头，或放入笼中6小时后断头。以3H-地西泮为配体评估大鼠大脑皮质和脑干中的苯二氮䓬受体结合情况。对这些数据进行Scatchard分析表明，无论是快速眼动睡眠剥夺还是快速眼动睡眠反弹，在体外均不影响3H-地西泮结合的动力学参数（最大结合容量Bmax或表观解离常数KD）。
