Erikson E, Erikson R L
Virology. 1983 Oct 15;130(1):221-6. doi: 10.1016/0042-6822(83)90130-7.
A widely cross reactive antiserum raised against denatured pp60v-src, the transforming protein encoded by Rous sarcoma virus, was used to test antigenic relationships with the transforming gene products encoded by other avian sarcoma viruses. The results showed that P90gag-yes, the transforming protein of a representative of Class III avian sarcoma viruses, is antigenically related to pp60v-src. Tryptic phosphopeptide analysis of P90gag-yes revealed two phosphotyrosine-containing peptides and one phosphoserine-containing peptide. One of the phosphotyrosine-containing peptides comigrated with the phosphotyrosine-containing tryptic peptide from pp60v-src.
一种针对变性的pp60v-src（劳氏肉瘤病毒编码的转化蛋白）产生的广泛交叉反应抗血清，被用于检测与其他禽肉瘤病毒编码的转化基因产物之间的抗原关系。结果显示，III类禽肉瘤病毒代表株的转化蛋白P90gag-yes与pp60v-src在抗原上相关。对P90gag-yes进行胰蛋白酶磷酸肽分析，发现了两个含磷酸酪氨酸的肽段和一个含磷酸丝氨酸的肽段。其中一个含磷酸酪氨酸的肽段与pp60v-src中含磷酸酪氨酸的胰蛋白酶肽段迁移率相同。
