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pp60src氨基端和羧基端区域特异性抗血清的研制与特性分析

Development and characterization of antisera specific for amino- and carboxy-terminal regions of pp60src.

作者信息

Resh M D, Erikson R L

出版信息

J Virol. 1985 Jul;55(1):242-5. doi: 10.1128/JVI.55.1.242-245.1985.

DOI:10.1128/JVI.55.1.242-245.1985
PMID:2409298
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC254921/
Abstract

Antisera to the Rous sarcoma virus transforming protein pp60src were produced in rabbits immunized with p60src purified from recombinant Escherichia coli. Immunization with isolated V8 proteolytic fragments of p60src resulted in antisera specific for the amino- and carboxy-terminal domains. The reactions of these antisera with pp60v-src and the normal cell protein p60c-src were characterized.

摘要

用从重组大肠杆菌中纯化的p60src免疫兔子，制备了抗劳斯肉瘤病毒转化蛋白pp60src的抗血清。用p60src分离的V8蛋白水解片段免疫，产生了对氨基末端和羧基末端结构域具有特异性的抗血清。对这些抗血清与pp60v-src和正常细胞蛋白p60c-src的反应进行了表征。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5300/254921/60bf731f8b47/jvirol00118-0254-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5300/254921/0ba656be3718/jvirol00118-0253-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5300/254921/37e2004185e0/jvirol00118-0253-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5300/254921/60bf731f8b47/jvirol00118-0254-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5300/254921/0ba656be3718/jvirol00118-0253-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5300/254921/37e2004185e0/jvirol00118-0253-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5300/254921/60bf731f8b47/jvirol00118-0254-a.jpg

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引用本文的文献

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本文引用的文献

1
A dot-immunobinding assay for monoclonal and other antibodies.一种用于单克隆抗体及其他抗体的斑点免疫结合测定法。
Anal Biochem. 1982 Jan 1;119(1):142-7. doi: 10.1016/0003-2697(82)90677-7.
2
Synthetic peptide fragment of src gene product inhibits the src protein kinase and crossreacts immunologically with avian onc kinases and cellular phosphoproteins.src基因产物的合成肽片段可抑制src蛋白激酶，并与禽肿瘤激酶和细胞磷蛋白发生免疫交叉反应。
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7412-6. doi: 10.1073/pnas.78.12.7412.
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Antibodies to a defined region of pp60src neutralize the tyrosine-specific kinase activity.
Mol Cell Biol. 1987 Apr;7(4):1450-8. doi: 10.1128/mcb.7.4.1450-1458.1987.
4
Reconstitution of the Rous sarcoma virus transforming protein pp60v-src into phospholipid vesicles.将劳氏肉瘤病毒转化蛋白pp60v-src重组到磷脂囊泡中。
Mol Cell Biol. 1988 May;8(5):1896-905. doi: 10.1128/mcb.8.5.1896-1905.1988.
5
Abnormal protein kinase C down regulation and reduced substrate levels in non-phorbol ester-responsive 3T3-TNR9 cells.在非佛波酯反应性3T3-TNR9细胞中蛋白激酶C异常下调及底物水平降低。
Mol Cell Biol. 1990 May;10(5):2122-32. doi: 10.1128/mcb.10.5.2122-2132.1990.
针对pp60src特定区域的抗体可中和酪氨酸特异性激酶活性。
J Biol Chem. 1983 Sep 25;258(18):11219-28.
4
Development of anti-pp60src serum with antigen produced in Escherichia coli.利用在大肠杆菌中产生的抗原制备抗pp60src血清。
J Virol. 1983 Jan;45(1):462-5. doi: 10.1128/JVI.45.1.462-465.1983.
5
Production of antibodies by inoculation into lymph nodes.通过接种到淋巴结中来产生抗体。
Methods Enzymol. 1983;93:3-12. doi: 10.1016/s0076-6879(83)93031-8.
6
Antigenic and structural studies on the transforming proteins of Rous sarcoma virus and Yamaguchi 73 avian sarcoma virus.劳氏肉瘤病毒和山口73禽肉瘤病毒转化蛋白的抗原性及结构研究。
Virology. 1983 Oct 15;130(1):221-6. doi: 10.1016/0042-6822(83)90130-7.
7
Isolation of monoclonal antibodies that recognize the transforming proteins of avian sarcoma viruses.识别禽肉瘤病毒转化蛋白的单克隆抗体的分离
J Virol. 1983 Nov;48(2):352-60. doi: 10.1128/JVI.48.2.352-360.1983.
8
Antiserum specific for the carboxy terminus of the transforming protein of Rous sarcoma virus.针对劳氏肉瘤病毒转化蛋白羧基末端的抗血清。
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9
DNA sequence of the viral and cellular src gene of chickens. 1. Complete nucleotide sequence of an EcoRI fragment of recovered avian sarcoma virus which codes for gp37 and pp60src.鸡的病毒和细胞src基因的DNA序列。1. 回收的禽肉瘤病毒的一个编码gp37和pp60src的EcoRI片段的完整核苷酸序列。
J Virol. 1982 Oct;44(1):1-11. doi: 10.1128/JVI.44.1.1-11.1982.
10
Immunofluorescent localization of the transforming protein of Rous sarcoma virus with antibodies against a synthetic src peptide.用针对合成src肽的抗体对劳氏肉瘤病毒转化蛋白进行免疫荧光定位。
Proc Natl Acad Sci U S A. 1982 Sep;79(17):5322-6. doi: 10.1073/pnas.79.17.5322.