Tomita T, Friesen S R, Kimmel J R, Doull V, Pollock H G
Am J Pathol. 1983 Nov;113(2):134-42.
Three cases of pancreatic islet cell tumors, 1 malignant and 2 benign, producing predominantly pancreatic polypeptide (PP) are described. All 3 patients exhibited elevated plasma PP concentrations, either basal or protein-meal-stimulated, during the period of observation. Immunocytochemical study revealed that while PP cells predominated in the tumor, A, B, and D cells were also present. A comparison of the hormone content of the tumor tissue, adjacent pancreatic tissue, and normal pancreas was made by radioimmunoassay of tissue extracts. The PP content of tumors clearly exceeded that of normal pancreas. The insulin, glucagon, and somatostatin (SRIF) content was more variable, but in one case the glucagon content of the tumor was higher than in normal pancreas, and two of the tumors exhibited an elevated SRIF content. Gel filtration of a tumor extract showed that insulin, glucagon, and PP immunoreactivity was of expected molecular dimensions but immunoreactive SRIF in this extract was composed of two species. The PP in gel fractions reacted equally well with antibody directed toward different parts of the PP molecule.
本文描述了三例胰岛细胞瘤，其中1例为恶性，2例为良性，主要分泌胰多肽（PP）。在观察期间，所有3例患者的血浆PP浓度，无论是基础浓度还是蛋白质餐刺激后的浓度均升高。免疫细胞化学研究显示，肿瘤中以PP细胞为主，但也存在A、B和D细胞。通过对组织提取物进行放射免疫测定，比较了肿瘤组织、邻近胰腺组织和正常胰腺的激素含量。肿瘤中的PP含量明显超过正常胰腺。胰岛素、胰高血糖素和生长抑素（SRIF）的含量变化更大，但在1例中肿瘤的胰高血糖素含量高于正常胰腺，且2例肿瘤的SRIF含量升高。对肿瘤提取物进行凝胶过滤显示，胰岛素、胰高血糖素和PP的免疫反应性具有预期的分子大小，但该提取物中的免疫反应性SRIF由两种成分组成。凝胶组分中的PP与针对PP分子不同部位的抗体反应同样良好。
