Panasenko O M, Azizova O A, Torkhovskaia T I, Dudaev V A, Vladimirov Iu A
Biokhimiia. 1983 Oct;48(10):1667-73.
The thermo-induced structural changes in lipoproteins (LP) of human plasma were investigated by the electron paramagnetic resonance method, using 5-doxylstearate as a spin probe. LP were shown to undergo thermotropic transformations at temperatures specific for each LP type. Differences were found in the nature of temperature dependences of the order parameter for the probe localized in LP and for isolated lipids. The denser the LP, the more pronounced the differences. 5-Doxylstearate was shown to be localized not only in the lipid phase of LP, but also in the area of the protein--lipid contact. This indicates that 5-doxylstearate is a useful tool for the study of protein--lipid interactions in pathological processes coupled with their modifications.
采用电子顺磁共振方法，以5 - 硬脂酸氧基为自旋探针，研究了人血浆脂蛋白（LP）的热诱导结构变化。结果表明，每种LP类型在特定温度下都会发生热致转变。对于位于LP中的探针和分离脂质，发现其序参数的温度依赖性性质存在差异。LP密度越高，差异越明显。5 - 硬脂酸氧基不仅定位于LP的脂质相，还定位于蛋白质 - 脂质接触区域。这表明5 - 硬脂酸氧基是研究病理过程中蛋白质 - 脂质相互作用及其修饰的有用工具。
