Sykes S E, Morgan A, Moores S R, Davison W, Beck J, Holmes A
Environ Health Perspect. 1983 Sep;51:267-73. doi: 10.1289/ehp.8351267.
The acute response of the rat lung to a range of fibrous materials has been investigated by bronchopulmonary lavage, at dose levels of 0.5 and 1.0 mg, 1 and 7 days after their administration by intratracheal instillation. The materials chosen for study included UICC chrysotile A, amosite, crocidolite and anthophyllite, and samples of S. African "long" amosite and glass fiber. In addition, the subacute response to 1, 2 and 3 mg of chrysotile and amosite has been studied at 50 and 100 days after instillation. In the acute phase at 1 day after instillation, the response to chrysotile was greater than that to any of the other materials, but by 7 days there was no gradation in the response to different dusts. In the subacute phase, cell recoveries were low, and it was not possible to assess the long-term cytotoxic or fibrogenic effects of amosite and chrysotile by analyses of lung washes, even though biochemical and histological methods indicated gross changes in lung pathology.
通过支气管肺泡灌洗，研究了大鼠肺对一系列纤维材料在气管内滴注给药后0.5毫克和1.0毫克剂量水平、给药后1天和7天的急性反应。所选研究材料包括国际癌症研究机构（UICC）的温石棉A、铁石棉、青石棉和直闪石，以及南非“长”铁石棉和玻璃纤维样本。此外，还研究了在滴注后50天和100天对1毫克、2毫克和3毫克温石棉和铁石棉的亚急性反应。在滴注后1天的急性期，对温石棉的反应大于对任何其他材料的反应，但到7天时，对不同粉尘的反应没有梯度差异。在亚急性期，细胞回收率较低，即使生化和组织学方法表明肺部病理学有明显变化，也无法通过分析肺灌洗来评估铁石棉和温石棉的长期细胞毒性或纤维生成作用。
