Brown R C, Chamberlain M, Griffiths D M, Timbrell V
Int J Cancer. 1978 Dec;22(6):721-7. doi: 10.1002/ijc.2910220614.
Three standard (UICC) samples of amphibole asbestos were subjected to ball-milling; the main effect of this procedure was to reduce the length of the fibres present in each sample. The numbers of fibres in unit masses, and the distribution of fibre sizes in all the samples, both parent and milled, were estimated from electron micrographs. The ability of all the samples to reduce the plating efficiency of V79-4 cells is compared, on the basis of mass, fibre number and fibre number number above various length thresholds. This biological activity of all the samples correlated best with the number of fibres above a threshold length of 6.5 micron. This is compared with the sizes of fibre previously reported to induce mesotheliomata when implanted into the pleural cavities of rats.
对三种标准的（国际癌症研究机构）闪石石棉样本进行了球磨处理；该过程的主要作用是缩短每个样本中纤维的长度。根据电子显微镜照片估算了所有样本（原始样本和球磨后样本）单位质量中的纤维数量以及纤维尺寸分布。基于质量、纤维数量以及高于各种长度阈值的纤维数量，比较了所有样本降低V79 - 4细胞铺板效率的能力。所有样本的这种生物活性与长度超过6.5微米阈值的纤维数量相关性最佳。将此与先前报道的植入大鼠胸腔时可诱发间皮瘤的纤维尺寸进行了比较。
